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Hippocampal asymmetry and regional dispersal of nAChRs alpha4 and alpha7 subtypes in the adult rat.
Baradaran, Raheleh; Anbarkeh, Fatemeh Rahimi; Delavar, Amir; Khorasgani, Elham Moghtadaei; Rahimian, Neda; Abbasi, Yusef; Jaberi, Najmeh.
Afiliación
  • Baradaran R; Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran. Electronic address: baradaranr941@mums.ac.ir.
  • Anbarkeh FR; Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Delavar A; Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Khorasgani EM; Department of Pathobiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • Rahimian N; Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran.
  • Abbasi Y; Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
  • Jaberi N; Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Chem Neuroanat ; 116: 101977, 2021 10.
Article en En | MEDLINE | ID: mdl-34052301
To better comprehend the relationship between left/right (L/R) differences and hippocampus functions is necessary knowledge of lateral asymmetry and regional distribution. This research was design to examine hippocampal L/R asymmetry and regional distribution profile of the alpha7 and alpha4 subtypes of nicotinic acetylcholine receptors (nAChRs) in the adult rat. 10-12-week-old twenty-four male wistar rats were randomly selected. After removing the brains, immunohistochemistry, real-time PCR, and western blot methods were applied to distinguish the presence of the receptors in the hippocampus. Outcomes stated that the mentioned receptors expression profile was spatial-dependent. As, the hippocampal dispersal of alpha7 and alpha4 subtypes in the left hippocampus (LH) was remarkably maximum compare with the right hippocampus (RH) (p = 0.001, p = 0.005 respectively). Furthermore, the alpha7 optical density (OD) was not significantly different in the diverse regions in hippocampus of adult rat (p = 0.057), while the maximum OD of the alpha4 was detected in the hippocampal dentate gyrus and CA3 regions of LH (p = 0.007, p = 0.009 respectively) and the minimum OD was in the CA1 of the RH (p = 0.019). In real time PCR evaluation, there is a significantly higher expression of alpha7 and alpha4 in LH compared to RH (p = 0.043, p = 0.049 respectively), also, for western blot (p = 0.042, p = 0.030 respectively). According to present data, the alpha7 and alpha4 nAChR subtypes expression profile demonstrated lateral asymmetry, the uniform regional dispersal for alpha7 and different regional dispersal for alpha4 in the adult rat hippocampus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Nicotínicos / Receptor Nicotínico de Acetilcolina alfa 7 / Hipocampo / Lateralidad Funcional Límite: Animals Idioma: En Revista: J Chem Neuroanat Asunto de la revista: ANATOMIA / NEUROLOGIA / QUIMICA Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Nicotínicos / Receptor Nicotínico de Acetilcolina alfa 7 / Hipocampo / Lateralidad Funcional Límite: Animals Idioma: En Revista: J Chem Neuroanat Asunto de la revista: ANATOMIA / NEUROLOGIA / QUIMICA Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos