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Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage.
Broderick, Michael T; Prince, Andrew D P; Dhukhwa, Asmita; Mukherjea, Debashree; Jiang, Peng; Campbell, Kathleen C M; Rybak, Leonard P; Brenner, Michael J.
Afiliación
  • Broderick MT; University of Michigan Medical School, Ann Arbor, Michigan.
  • Prince ADP; University of Michigan Medical School, Ann Arbor, Michigan.
  • Dhukhwa A; Department of Pharmacology, School of Medicine, Southern Illinois University.
  • Mukherjea D; Department of Otolaryngology-Head and Neck Surgery and Department of Pharmacology, Southern Illinois University Schools of Medicine, Springfield, Illinois.
  • Jiang P; Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  • Campbell KCM; Department of Otolaryngology-Head and Neck Surgery and Department of Pharmacology, Southern Illinois University Schools of Medicine, Springfield, Illinois.
  • Rybak LP; Department of Medical Microbiology, Immunology, and Cell Biology, and Department of Pharmacology, Southern Illinois University Schools of Medicine, Springfield, Illinois.
  • Brenner MJ; Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
Otol Neurotol ; 42(8): 1261-1268, 2021 09 01.
Article en En | MEDLINE | ID: mdl-34049329
HYPOTHESIS: Moringa extract, a naturally occurring anti-oxidant, protects against aminoglycoside-induced hair cell death and hearing loss within the organ of Corti. BACKGROUND: Reactive oxygen species (ROS) arise primarily in the mitochondria and have been implicated in aminoglycoside-induced ototoxicity. Mitochondrial dysfunction results in loss of membrane potential, release of caspases, and cell apoptosis. Moringa extract has not previously been examined as a protective agent for aminoglycoside-induced ototoxicity. METHODS: Putative otoprotective effects of moringa extract were investigated in an organotypic model using murine organ of Corti explants subjected to gentamicin-induced ototoxicity. Assays evaluated hair cell loss, cytochrome oxidase expression, mitochondrial membrane potential integrity, and caspase activity. RESULTS: In vitro application of moringa conferred significant protection from gentamicin-induced hair cell loss at dosages from 25 to 300 µg/mL, with dosages above 100 µg/mL conferring near complete protection. Assays demonstrated moringa extract suppression of ROS, preservation of cytochrome oxidase activity, and reduction in caspase production. CONCLUSION: Moringa extract demonstrated potent antioxidant properties with significant protection against gentamicin ototoxicity in cochlear explants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Moringa / Aminoglicósidos Límite: Animals Idioma: En Revista: Otol Neurotol Asunto de la revista: NEUROLOGIA / OTORRINOLARINGOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Moringa / Aminoglicósidos Límite: Animals Idioma: En Revista: Otol Neurotol Asunto de la revista: NEUROLOGIA / OTORRINOLARINGOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos