Your browser doesn't support javascript.
loading
Role of microRNA­375­3p­mediated regulation in tinnitus development.
Han, Kyu-Hee; Cho, Hyeeun; Han, Kyeo-Rye; Mun, Seog-Kyun; Kim, Young-Kook; Park, Ilyong; Chang, Munyoung.
Afiliación
  • Han KH; Department of Otorhinolaryngology­Head and Neck Surgery, National Medical Center, Seoul 04564, Republic of Korea.
  • Cho H; Department of Otorhinolaryngology­Head and Neck Surgery, Chung­Ang University College of Medicine, Seoul 06974, Republic of Korea.
  • Han KR; Department of Otorhinolaryngology­Head and Neck Surgery, Chung­Ang University College of Medicine, Seoul 06974, Republic of Korea.
  • Mun SK; Department of Otorhinolaryngology­Head and Neck Surgery, Chung­Ang University College of Medicine, Seoul 06974, Republic of Korea.
  • Kim YK; Department of Biochemistry, Chonnam National University Medical School, Hwasun, Jeollanam-do 58128, Republic of Korea.
  • Park I; Department of Biomedical Engineering, Dankook University College of Medicine, Cheonan, Chungcheongnam-do 16890, Republic of Korea.
  • Chang M; Department of Otorhinolaryngology­Head and Neck Surgery, Chung­Ang University College of Medicine, Seoul 06974, Republic of Korea.
Int J Mol Med ; 48(1)2021 Jul.
Article en En | MEDLINE | ID: mdl-34036397
Changes in the dorsal cochlear nucleus (DCN) following exposure to noise play an important role in the development of tinnitus. As the development of several diseases is known to be associated with microRNAs (miRNAs/miRs), the aim of the present study was to identify the miRNAs that may be implicated in pathogenic changes in the DCN, resulting in tinnitus. A previously developed tinnitus animal model was used for this study. The study consisted of four stages, including identification of candidate miRNAs involved in tinnitus development using miRNA microarray analysis, validation of miRNA expression using reverse transcription­quantitative PCR (RT­qPCR), evaluation of the effects of candidate miRNA overexpression on tinnitus development through injection of a candidate miRNA mimic or mimic negative control, and target prediction of candidate miRNAs using mRNA microarray analysis and western blotting. The miRNA microarray and RT­qPCR analyses revealed that miR­375­3p expression was significantly reduced in the tinnitus group compared with that in the non­tinnitus group. Additionally, miR­375­3p overexpression via injection of miR­375­3p mimic reduced the proportion of animals with persistent tinnitus. Based on mRNA microarray and western blot analyses, connective tissue growth factor (CTGF) was identified as a potential target for miR­375­3p. Thus, it was inferred that CTGF downregulation by miR­375­3p may weaken with the decrease in miRNA expression, and the increased pro­apoptotic activity of CTGF may result in more severe neuronal damage, contributing to tinnitus development. These findings are expected to contribute significantly to the development of a novel therapeutic approach to tinnitus, thereby bringing about a significant breakthrough in the treatment of this potentially debilitating condition.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acúfeno / Regulación de la Expresión Génica / MicroARNs / Factor de Crecimiento del Tejido Conjuntivo Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Med Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2021 Tipo del documento: Article Pais de publicación: Grecia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acúfeno / Regulación de la Expresión Génica / MicroARNs / Factor de Crecimiento del Tejido Conjuntivo Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Med Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2021 Tipo del documento: Article Pais de publicación: Grecia