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Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq.
Yelagandula, Ramesh; Bykov, Aleksandr; Vogt, Alexander; Heinen, Robert; Özkan, Ezgi; Strobl, Marcus Martin; Baar, Juliane Christina; Uzunova, Kristina; Hajdusits, Bence; Kordic, Darja; Suljic, Erna; Kurtovic-Kozaric, Amina; Izetbegovic, Sebija; Schaeffer, Justine; Hufnagl, Peter; Zoufaly, Alexander; Seitz, Tamara; Födinger, Manuela; Allerberger, Franz; Stark, Alexander; Cochella, Luisa; Elling, Ulrich.
Afiliación
  • Yelagandula R; Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC), Vienna, Austria.
  • Bykov A; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
  • Vogt A; Vienna Biocenter Core Facilities GmbH (VBCF), Vienna, Austria.
  • Heinen R; Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC), Vienna, Austria.
  • Özkan E; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
  • Strobl MM; Gregor Mendel Institute (GMI), Vienna BioCenter (VBC), Vienna, Austria.
  • Baar JC; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
  • Uzunova K; Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC), Vienna, Austria.
  • Hajdusits B; Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC), Vienna, Austria.
  • Kordic D; Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC), Vienna, Austria.
  • Suljic E; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
  • Kurtovic-Kozaric A; Gregor Mendel Institute (GMI), Vienna BioCenter (VBC), Vienna, Austria.
  • Izetbegovic S; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
  • Schaeffer J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.
  • Hufnagl P; Clinical Center of the University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
  • Zoufaly A; Clinical Center of the University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
  • Seitz T; Clinical Center of the University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
  • Födinger M; EUPHEM Fellowship, European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden.
  • Allerberger F; Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES), Vienna, Austria.
  • Stark A; Department for Infectious Diseases, Clinic Favoriten, Vienna, Austria.
  • Cochella L; Faculty of Medicine, Sigmund Freud Private University, Vienna, Austria.
  • Elling U; Department for Infectious Diseases, Clinic Favoriten, Vienna, Austria.
Nat Commun ; 12(1): 3132, 2021 05 25.
Article en En | MEDLINE | ID: mdl-34035246
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands of samples in parallel. SARSeq relies on next generation sequencing of multiple amplicons generated in a multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous and scalable assignment of reads to individual samples. We calibrate SARSeq on SARS-CoV-2 synthetic RNA, virions, and hundreds of human samples of various types. Robustness and sensitivity were virtually identical to quantitative RT-PCR. Double-blinded benchmarking to gold standard quantitative-RT-PCR performed by human diagnostics laboratories confirms this high sensitivity. SARSeq can be used to detect Influenza A and B viruses and human rhinovirus in parallel, and can be expanded for detection of other pathogens. Thus, SARSeq is ideally suited for differential diagnostic of infections during a pandemic.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones del Sistema Respiratorio / Virus / Técnicas de Laboratorio Clínico / Ensayos Analíticos de Alto Rendimiento Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones del Sistema Respiratorio / Virus / Técnicas de Laboratorio Clínico / Ensayos Analíticos de Alto Rendimiento Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Reino Unido