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S-nitrosated proteomic analysis reveals the regulatory roles of protein S-nitrosation and S-nitrosoglutathione reductase during Al-induced PCD in peanut root tips.
Pan, Chunliu; Li, Xia; Yao, Shaochang; Luo, Shuzhen; Liu, Songying; Wang, Aiqin; Xiao, Dong; Zhan, Jie; He, Longfei.
Afiliación
  • Pan C; College of Agronomy, Guangxi University, Nanning, China; Guangxi Botanical Garden of Medicinal Plants, Nanning, China; College of Life Science and Technology, Guangxi University, Nanning, China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning, China.
  • Li X; College of Agronomy, Guangxi University, Nanning, China.
  • Yao S; College of Agronomy, Guangxi University, Nanning, China.
  • Luo S; College of Agronomy, Guangxi University, Nanning, China.
  • Liu S; College of Agronomy, Guangxi University, Nanning, China.
  • Wang A; College of Agronomy, Guangxi University, Nanning, China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning, China.
  • Xiao D; College of Agronomy, Guangxi University, Nanning, China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning, China.
  • Zhan J; College of Agronomy, Guangxi University, Nanning, China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning, China. Electronic address: may2399@163.com.
  • He L; College of Agronomy, Guangxi University, Nanning, China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning, China. Electronic address: lfhe@gxu.edu.cn.
Plant Sci ; 308: 110931, 2021 Jul.
Article en En | MEDLINE | ID: mdl-34034861
Nitric oxide-mediated S-nitrosation through S-nitrosoglutathione reductase (GSNOR) plays important roles in cellular processes and signaling of plants; however, the regulatory mechanism of programmed cell death (PCD) by S-nitrosation remains unclear. In this study, the S-nitrosated proteomic and functions of GSNOR during Al-induced PCD in peanut were investigated. Al stress induced an increase of S-nitrosothiol (SNO) content and GSNOR activity in Al-induced PCD. There was significant positive correlation between SNO content and hydrogen peroxide content. The S-nitrosated proteomic analysis identified 402 S-nitrosated proteins containing 551 S-nitrosated sites during Al-induced PCD in the root tips of peanut. These S-nitrosated proteins were involved in regulation of various biological processes including energy metabolism, maintenance of cell wall function and organic acid secretion. Among them, 128 S-nitrosated proteins were up-regulated and one was down-regulated after Al stress. Experiments with recombinant AhGSNOR revealed that activity of the enzyme was inhibited by its S-nitrosation, with a moderate decrease of 17.9 % after 100 µM GSNO incubation. These data provide novel insights to understanding the functional mechanism of NO-mediated S-nitrosation during plant PCD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arachis / Proteínas de Plantas / Meristema / Proteoma / Aldehído Oxidorreductasas / Aluminio Idioma: En Revista: Plant Sci Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Irlanda
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arachis / Proteínas de Plantas / Meristema / Proteoma / Aldehído Oxidorreductasas / Aluminio Idioma: En Revista: Plant Sci Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Irlanda