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Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile.
Zhang, Na; Sun, An-di; Sun, Si-Man; Yang, Rui; Shi, Yan-Yan; Wang, Qi-Long; Li, Xin-Yu; Ma, Ji-Hong; Yue, Wei; Xie, Bing-Teng; Qiao, Jie; Li, Mo.
Afiliación
  • Zhang N; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.
  • Sun AD; National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, 100191, China.
  • Sun SM; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, 100191, China.
  • Yang R; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
  • Shi YY; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.
  • Wang QL; National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, 100191, China.
  • Li XY; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, 100191, China.
  • Ma JH; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China.
  • Yue W; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.
  • Xie BT; Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.
  • Qiao J; National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, 100191, China.
  • Li M; Research Center of Clinical Epidemiology, Peking University Third Hospital, Beijing, 100191, China.
Acta Pharmacol Sin ; 42(12): 2144-2154, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34017067
Mitochondria are essential organelles that provide energy for mammalian cells and participate in multiple functions, such as signal transduction, cellular differentiation, and regulation of apoptosis. Compared with the mitochondria in somatic cells, oocyte mitochondria have an additional level of importance since they are required for germ cell maturation, dysfunction in which can lead to severe inherited disorders. Thus, a systematic proteomic profile of oocyte mitochondria is urgently needed to support the basic and clinical research, but the acquisition of such a profile has been hindered by the rarity of oocyte samples and technical challenges associated with capturing mitochondrial proteins from live oocytes. Here, in this work, using proximity labeling proteomics, we established a mitochondria-specific ascorbate peroxidase (APEX2) reaction in live GV-stage mouse oocytes and identified a total of 158 proteins in oocyte mitochondria. This proteome includes intrinsic mitochondrial structural and functional components involved in processes associated with "cellular respiration", "ATP metabolism", "mitochondrial transport", etc. In addition, mitochondrial proteome capture after oocyte exposure to the antitumor chemotherapeutic cisplatin revealed differential changes in the abundance of several oocyte-specific mitochondrial proteins. Our study provides the first description of a mammalian oocyte mitochondrial proteome of which we are aware, and further illustrates the dynamic shifts in protein abundance associated with chemotherapeutic agents.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Cisplatino / Proteoma / Proteínas Mitocondriales / Mitocondrias / Antineoplásicos Límite: Animals Idioma: En Revista: Acta Pharmacol Sin Asunto de la revista: FARMACOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Cisplatino / Proteoma / Proteínas Mitocondriales / Mitocondrias / Antineoplásicos Límite: Animals Idioma: En Revista: Acta Pharmacol Sin Asunto de la revista: FARMACOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos