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Dentin Cross-linking Effect of Carbodiimide After 5 Years.
Maravic, T; Mancuso, E; Comba, A; Checchi, V; Generali, L; Mazzitelli, C; Josic, U; Hass, V; Reis, A; Loguercio, A D; Tay, F R; Breschi, L; Mazzoni, A.
Afiliación
  • Maravic T; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
  • Mancuso E; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
  • Comba A; Department of Surgical Sciences, University of Turin, Torino, Italy.
  • Checchi V; Unit of Dentistry and Oral-maxillo-facial Surgery, Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy.
  • Generali L; Unit of Dentistry and Oral-maxillo-facial Surgery, Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy.
  • Mazzitelli C; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
  • Josic U; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
  • Hass V; School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
  • Reis A; School of Dentistry, Oral and Craniofacial Sciences, University of Missouri-Kansas City, Kansas City, Missouri, USA.
  • Loguercio AD; Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Ponta Grossa, Brazil.
  • Tay FR; Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Ponta Grossa, Brazil.
  • Breschi L; The Dental College of Georgia, Augusta University, Augusta, GA, USA.
  • Mazzoni A; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
J Dent Res ; 100(10): 1090-1098, 2021 09.
Article en En | MEDLINE | ID: mdl-34009064
Carbodiimide (EDC)-based dentin primers preserve hybrid layer (HL) integrity. However, aging >1 y has not been investigated. The present study examined whether the cross-linking effect of EDC was reflected in dentin bond strength, endogenous enzymatic activity, and the chemical profile of the HL after 5-y aging in artificial saliva. Noncarious human third molars (N = 42) were cut to expose middle/deep coronal dentin and treated as follows: group 1, dentin etched with 35% H3PO4, pretreated with a 0.3M aqueous EDC primer for 1 min and restored with XP Bond (Dentsply Sirona); group 2, as in group 1 but without EDC pretreatment; group 3, Clearfil SE Bond (Kuraray-Noritake) primer applied to dentin surface, followed by EDC pretreatment as in group 1 and application of bond; group 4, as in group 3 without EDC pretreatment. After composite buildup, the specimens were cut into sticks or slabs, depending on the experiment. All tests were performed at baseline (T0) and after 5 y of aging (T5) in artificial saliva at 37 °C. Microtensile bond strength (µTBS) was tested at a crosshead speed of 1 mm/min until failure. Endogenous enzymatic activity was investigated with in situ zymography. The chemical profile of HL was determined via Raman spectroscopy. Three-way analysis of variance and post hoc Tukey test were used to analyze µTBS and in situ zymography data (α = 0.05). EDC pretreatment and aging significantly influenced µTBS and in situ zymography results (P < 0.05). Higher bond strength and lower gelatinolytic activity were identified in the EDC-treated groups at T5 (P < 0.05), especially in the etch-and-rinse groups. Raman spectra revealed less defined amide III peaks in control specimens at T5. The EDC cross-linking effect persisted in the HL for 5 y in terms of bond strength, collagen structure preservation, and dentinal enzyme silencing.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbodiimidas / Recubrimiento Dental Adhesivo Límite: Humans Idioma: En Revista: J Dent Res Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbodiimidas / Recubrimiento Dental Adhesivo Límite: Humans Idioma: En Revista: J Dent Res Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos