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Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection.
El-Mokhtar, Mohamed A; Karam-Allah Ramadan, Haidi; Abdel Hameed, Muhamad R; M Kamel, Ayat; A Mandour, Sahar; Ali, Maha; Abdel-Malek, Mohamed A Y; M Abd El-Kareem, Doaa; Adel, Sara; H Salama, Eman; Khalaf, Khaled Abo Bakr; Sayed, Ibrahim M.
Afiliación
  • El-Mokhtar MA; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
  • Karam-Allah Ramadan H; Microbiology and Immunology Department, Faculty of Pharmacy, Sphinx University, Assiut, Egypt.
  • Abdel Hameed MR; Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.
  • M Kamel A; Department of Internal Medicine and Hematology Unit, Assiut University Hospitals, Assiut University, Assiut, Egypt.
  • A Mandour S; Microbiology and Immunology Department, Faculty of Pharmacy, Assiut University, Assiut Egypt.
  • Ali M; Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia, Egypt.
  • Abdel-Malek MAY; Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt.
  • M Abd El-Kareem D; Department of Clinical Pathology, Faculty of Medicine, Assiut University, Egypt.
  • Adel S; Department of Clinical Pathology, Faculty of Medicine, Assiut University, Egypt.
  • H Salama E; Department of Clinical Pathology, Faculty of Medicine, Al-Azhar University, Assiut, Egypt.
  • Khalaf KAB; Department of Clinical Pathology, Faculty of Medicine, Sohag University, Sohag, Egypt.
  • Sayed IM; Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Virulence ; 12(1): 1334-1344, 2021 12.
Article en En | MEDLINE | ID: mdl-34002677
HEV-Ag ELISA assay is a reliable diagnostic test in resource-limited areas. HEV genotype 1 (HEV-1) infections are either self-limited or progress to fulminant hepatic failure (FHF) and death if anti-HEV therapy is delayed. Limited data is available about the diagnostic utility of HEV Ag on HEV-1 infections. Herein wWe aimed to study the kinetics of HEV Ag during HEV-1 infections at different stages, i.e., acute HEV infection, recovery, and progression to FHF. Also, we evaluated the diagnostic utility of this marker to predict the outcomes of HEV-1 infections. Plasma of acute hepatitis E (AHE) patients were assessed for HEV RNA by RT-qPCR, HEV Ag, and anti-HEV IgM by ELISA. The kinetics of HEV Ag was monitored at different time points; acute phase of infection, recovery, FHF stage, and post-recovery. Our results showed that the level of HEV Ag was elevated in AHE patients with a significantly higher level in FHF patients than recovered patients. We identified a plasma HEV Ag threshold that can differentiate between self-limiting infection and FHF progression with 100% sensitivity and 88.89% specificity. HEV Ag and HEV RNA have similar kinetics during the acute phase and self-limiting infection. In the FHF stage, HEV Ag and anti-HEV IgM have similar patterns of kinetics which could be the cause of liver damage. In conclusion, the HEV Ag assay can be used as a biomarker for predicting the consequences of HEV-1 infections which could be diagnostically useful for taking the appropriate measures to reduce the complications, especially for high-risk groups.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Hepatitis E / Hepatitis E / Antígenos de la Hepatitis Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Virulence Año: 2021 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Hepatitis E / Hepatitis E / Antígenos de la Hepatitis Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Virulence Año: 2021 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Estados Unidos