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Halogenation of estrogens catalysed by a fungal chloroperoxidase.
Undiano, Elizabeth; Roman, Rosa; Miranda-Molina, Alfonso; Ayala, Marcela.
Afiliación
  • Undiano E; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, Mexico.
  • Roman R; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, Mexico.
  • Miranda-Molina A; LANEM-Centro de Investigaciones Químicas-IICBA, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
  • Ayala M; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, Mexico.
Nat Prod Res ; 36(20): 5353-5357, 2022 Oct.
Article en En | MEDLINE | ID: mdl-34000929
Chloroperoxidase (CPO) is a haeme-thiolate enzyme able to catalyse the halogenation and oxidation of a wide range of organic substrates. In this work, the CPO-catalysed chlorination and bromination reaction of natural estrogens was characterised. Estradiol, estrone and equiline were efficiently converted to halogenated compounds in the presence of chloride or bromide and hydrogen peroxide. The catalytic efficiency of CPO in this reaction is similar to that measured for other aromatic substrates; as expected the bromination reaction proceeds more efficiently than the chlorination reaction. Three major products were detected for chlorination of estradiol; two of them were monohalogenated compounds while a third product was a dihalogenated compound at positions 2 and 4 of the aromatic ring A. Chlorinated compounds are not substrates for tyrosinase, suggesting that the halogenated form of estrogens is less susceptible to form o-quinones.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cloruro Peroxidasa Idioma: En Revista: Nat Prod Res Año: 2022 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cloruro Peroxidasa Idioma: En Revista: Nat Prod Res Año: 2022 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido