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Genetic diversity of Francisella tularensis subsp. holarctica in Kazakhstan.
Shevtsov, Vladislav; Kairzhanova, Alma; Shevtsov, Alexandr; Shustov, Alexandr; Kalendar, Ruslan; Abdrakhmanov, Sarsenbay; Lukhnova, Larissa; Izbanova, Uinkul; Ramankulov, Yerlan; Vergnaud, Gilles.
Afiliación
  • Shevtsov V; National Center for Biotechnology, Nur Sultan, Kazakhstan.
  • Kairzhanova A; National Center for Biotechnology, Nur Sultan, Kazakhstan.
  • Shevtsov A; S. Seifullin Kazakh Agrotechnical University, Nur Sultan, Kazakhstan.
  • Shustov A; National Center for Biotechnology, Nur Sultan, Kazakhstan.
  • Kalendar R; National Center for Biotechnology, Nur Sultan, Kazakhstan.
  • Abdrakhmanov S; National Center for Biotechnology, Nur Sultan, Kazakhstan.
  • Lukhnova L; S. Seifullin Kazakh Agrotechnical University, Nur Sultan, Kazakhstan.
  • Izbanova U; National Scientific Center for Especially Dangerous Infections named by Masgut Aykimbayev, Almaty, Kazakhstan.
  • Ramankulov Y; National Scientific Center for Especially Dangerous Infections named by Masgut Aykimbayev, Almaty, Kazakhstan.
  • Vergnaud G; National Center for Biotechnology, Nur Sultan, Kazakhstan.
PLoS Negl Trop Dis ; 15(5): e0009419, 2021 05.
Article en En | MEDLINE | ID: mdl-33999916
Tularemia is a highly dangerous zoonotic infection due to the bacteria Francisella tularensis. Low genetic diversity promoted the use of polymorphic tandem repeats (MLVA) as first-line assay for genetic description. Whole genome sequencing (WGS) is becoming increasingly accessible, opening the perspective of a time when WGS might become the universal genotyping assay. The main goal of this study was to describe F. tularensis strains circulating in Kazakhstan based on WGS data and develop a MLVA assay compatible with in vitro and in silico analysis. In vitro MLVA genotyping and WGS were performed for the vaccine strain and for 38 strains isolated in Kazakhstan from natural water bodies, ticks, rodents, carnivores, and from one migratory bird, an Isabellina wheatear captured in a rodent burrow. The two genotyping approaches were congruent and allowed to attribute all strains to two F. tularensis holarctica lineages, B.4 and B.12. The seven tandem repeats polymorphic in the investigated strain collection could be typed in a single multiplex PCR assay. Identical MLVA genotypes were produced by in vitro and in silico analysis, demonstrating full compatibility between the two approaches. The strains from Kazakhstan were compared to all publicly available WGS data of worldwide origin by whole genome SNP (wgSNP) analysis. Genotypes differing at a single SNP position were collected within a time interval of more than fifty years, from locations separated from each other by more than one thousand kilometers, supporting a role for migratory birds in the worldwide spread of the bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tularemia / Francisella Límite: Animals País/Región como asunto: Asia / Europa Idioma: En Revista: PLoS Negl Trop Dis Asunto de la revista: MEDICINA TROPICAL Año: 2021 Tipo del documento: Article País de afiliación: Kazajstán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tularemia / Francisella Límite: Animals País/Región como asunto: Asia / Europa Idioma: En Revista: PLoS Negl Trop Dis Asunto de la revista: MEDICINA TROPICAL Año: 2021 Tipo del documento: Article País de afiliación: Kazajstán Pais de publicación: Estados Unidos