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Performance study of a sterilization box using a combination of heat and ultraviolet light irradiation for the prevention of COVID-19.
Mahanta, Nilkamal; Saxena, Varun; Pandey, Lalit M; Batra, Priyanka; Dixit, U S.
Afiliación
  • Mahanta N; Department of Mechanical Engineering, Indian Institute of Technology Guwahati, India.
  • Saxena V; Bio-Interface and Environmental Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India.
  • Pandey LM; Bio-Interface and Environmental Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India.
  • Batra P; North East Center for Biological Sciences and Health Care Engineering (NECBH), Indian Institute of Technology Guwahati, India.
  • Dixit US; Department of Mechanical Engineering, Indian Institute of Technology Guwahati, India. Electronic address: uday@iitg.ac.in.
Environ Res ; 198: 111309, 2021 07.
Article en En | MEDLINE | ID: mdl-33984307
SARS-CoV-2 virus and other pathogenic microbes are transmitted to the environment through contacting surfaces, which need to be sterilized for the prevention of COVID-19 and related diseases. In this study, a prototype of a cost-effective sterilization box is developed to disinfect small items. The box utilizes ultra violet (UV) radiation with heat. For performance assessment, two studies were performed. First, IgG (glycoprotein, a model protein similar to that of spike glycoprotein of SARS-COV-2) was incubated under UV and heat sterilization. An incubation with UV at 70 °C for 15 min was found to be effective in unfolding and aggregation of the protein. At optimized condition, the hydrodynamic size of the protein increased to ~171 nm from ~5 nm of the native protein. Similarly, the OD280 values also increased from 0.17 to 0.78 indicating the exposure of more aromatic moieties and unfolding of the protein. The unfolding and aggregation of the protein were further confirmed by the intrinsic fluorescence measurement and FTIR studies, showing a 70% increase in the ß-sheets and a 22% decrease in the α-helixes of the protein. The designed box was effective in damaging the protein's native structure indicating the effective inactivation of the SARS-COV-2. Furthermore, the incubation at 70 °C for 15 min inside the chamber resulted in 100% antibacterial efficacy for the clinically relevant E.coli bacteria as well as for bacteria collected from daily use items. It is the first detailed performance study on the efficacy of using UV irradiation and heat together for disinfection from virus and bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rayos Ultravioleta / COVID-19 Límite: Humans Idioma: En Revista: Environ Res Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rayos Ultravioleta / COVID-19 Límite: Humans Idioma: En Revista: Environ Res Año: 2021 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos