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Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of Encephalitozoon cuniculi.
Vecková, Tereza; Sak, Bohumil; Samková, Eva; Holubová, Nikola; Kicia, Marta; Zajaczkowska, Zaneta; Hlásková, Lenka; Kvetonová, Dana; Kvác, Martin.
Afiliación
  • Vecková T; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Sak B; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Ceské Budejovice, Czech Republic.
  • Samková E; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Holubová N; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Ceské Budejovice, Czech Republic.
  • Kicia M; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
  • Zajaczkowska Z; Faculty of Agriculture, University of South Bohemia in Ceské Budejovice, Ceské Budejovice, Czech Republic.
  • Hlásková L; Department of Biology and Medical Parasitology, Wroclaw Medical University, Wroclaw, Poland.
  • Kvetonová D; Department of Biology and Medical Parasitology, Wroclaw Medical University, Wroclaw, Poland.
  • Kvác M; Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceské Budejovice, Czech Republic.
Foodborne Pathog Dis ; 18(9): 661-667, 2021 09.
Article en En | MEDLINE | ID: mdl-33978456
The aim of this work was to evaluate the effect of pasteurization and coagulation during goat cheese production on the infectivity of Encephalitozoon cuniculi spores for immunodeficient (SCID, CD4-/-, and CD8-/-) and immunocompetent (BALB/c and C57BL/6) mice. Goat milk and fecal samples were screened for the presence and quantity of microsporidial DNA using molecular methods. Experimentally produced cheese from E. cuniculi-enriched goat milk or goat cheese purchased from retail producers was fed with experimental mice susceptible to E. cuniculi infection. The mice were sacrificed in the presumed acute phase of infection and samples of their tissues were subject to molecular detection of specific E. cuniculi DNA. Specific DNA of E. cuniculi genotype II was detected in feces and milk of three out of 99 goats kept on 6 farms in the Czech Republic. Under experimental conditions, spores of E. cuniculi genotype II remained viable in artificially enriched fresh cheese and were able to cause infection in laboratory mice. E. cuniculi genotype I and II DNA were detected in eight of the nine goat cheeses purchased from various producers/breeders in the Czech Republic and these cheeses were able to develop infection in both immunodeficient and immunocompetent mice. The results of these experiments showed that spores of E. cuniculi genotype I and II are able to remain viable after cheese processing and thus fresh and soft cheeses should be considered a potential source of microsporidia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queso / Encefalitozoonosis / Encephalitozoon cuniculi Límite: Animals Idioma: En Revista: Foodborne Pathog Dis Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queso / Encefalitozoonosis / Encephalitozoon cuniculi Límite: Animals Idioma: En Revista: Foodborne Pathog Dis Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos