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Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1-Related Microglial Activation in Neonatal Hypoxic-Ischemic Encephalopathy: Morphologic Consideration.
Akamatsu, Tomohisa; Sugiyama, Takehiro; Oshima, Takuya; Aoki, Yoshinori; Mizukami, Ayumi; Goishi, Keiji; Shichino, Hiroyuki; Kato, Norihiro; Takahashi, Naoto; Goto, Yu-Ichi; Oka, Akira; Itoh, Masayuki.
Afiliación
  • Akamatsu T; Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan; Department of Pediatrics, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan; Department of Gene Diagnostics and Thera
  • Sugiyama T; Diabetes and Metabolism Information Center, National Center for Global Health and Medicine, Tokyo, Japan; Research Institute, Institute for Global Health Policy Research, Bureau of International Health Cooperation, National Center for Global Health and Medicine, Tokyo, Japan; Department of Health Se
  • Oshima T; Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
  • Aoki Y; Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
  • Mizukami A; Department of Pediatrics, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.
  • Goishi K; Department of Pediatrics, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.
  • Shichino H; Department of Pediatrics, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.
  • Kato N; Department of Gene Diagnostics and Therapeutics, National Center for Global Health and Medicine, Tokyo, Japan.
  • Takahashi N; Department of Pediatrics, the University of Tokyo Hospital, Tokyo, Japan.
  • Goto YI; Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
  • Oka A; Department of Pediatrics, the University of Tokyo Hospital, Tokyo, Japan.
  • Itoh M; Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. Electronic address: itoh@ncnp.go.jp.
Am J Pathol ; 191(7): 1303-1313, 2021 07.
Article en En | MEDLINE | ID: mdl-33964218
Neonatal hypoxic-ischemic encephalopathy (nHIE) is a major neonatal brain injury. Despite therapeutic hypothermia, mortality and sequelae remain severe. The lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is associated with the pathophysiology of nHIE. In this study, morphologic change and microglial activation under the nHIE condition and LOX-1 treatment were investigated. The microglial activity and proliferation were assessed with a novel morphologic method, immunostaining, and quantitative PCR in the rat brains of both nHIE model and anti-LOX-1 treatment. Circumference ratio, the long diameter ratio, the cell area ratio, and the roundness of microglia were calculated. The correlation of the morphologic metrics and microglial activation in nHIE model and anti-LOX-1 treated brains was evaluated. LOX-1 was expressed in activated ameboid and round microglia in the nHIE model rat brain. In the evaluation of microglial activation, the novel morphologic metrics correlated with all scales of the nHIE-damaged and treated brains. While the circumference and long diameter ratios had a positive correlation, the cell area ratio and roundness had a negative correlation. Anti-LOX-1 treatment attenuated morphologic microglial activation and proliferation, and suppressed the subsequent production of inflammatory mediators by microglia. In human nHIE, round microglia and endothelial cells expressed LOX-1. The results indicate that LOX-1 regulates microglial activation in nHIE and anti-LOX-1 treatment attenuates brain injury by suppressing microglial activation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microglía / Hipoxia-Isquemia Encefálica / Receptores Depuradores de Clase E Límite: Animals / Humans Idioma: En Revista: Am J Pathol Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microglía / Hipoxia-Isquemia Encefálica / Receptores Depuradores de Clase E Límite: Animals / Humans Idioma: En Revista: Am J Pathol Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos