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Evaluation of two sexual-stage antigens as bivalent transmission-blocking vaccines in rodent malaria.
Yang, Fan; Liu, Fei; Yu, Xinxin; Zheng, Wenqi; Wu, Yudi; Qiu, Yue; Jin, Ying; Cui, Liwang; Cao, Yaming.
Afiliación
  • Yang F; Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110122, Liaoning, China.
  • Liu F; Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110122, Liaoning, China.
  • Yu X; Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110122, Liaoning, China.
  • Zheng W; Department of Laboratory Medicine, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.
  • Wu Y; Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110122, Liaoning, China.
  • Qiu Y; The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China.
  • Jin Y; Liaoning Research Institute of Family Planning, Shenyang, 110031, China.
  • Cui L; Department of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
  • Cao Y; Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110122, Liaoning, China. ymcao@cmu.edu.cn.
Parasit Vectors ; 14(1): 241, 2021 May 07.
Article en En | MEDLINE | ID: mdl-33962671
BACKGROUND: Transmission-blocking vaccine (TBV) is a promising strategy for malaria elimination. It is hypothesized that mixing or fusing two antigens targeting different stages of sexual development may provide higher transmission-blocking activity than these antigens used individually. METHODS: A chimeric protein composed of fragments of Pbg37 and PSOP25 was designed and expressed the recombinant protein in Escherichia coli Rosetta-gami B (DE3). After immunizing mice with individual recombinant proteins Pbg37 and PSOP25, mixed proteins (Pbg37+PSOP25), or the fusion protein (Pbg37-PSOP25), the antibody titers of individual sera were analyzed by ELISA. IFA and Western blot were performed to test the reactivity of the antisera with the native proteins in the parasite. The transmission-blocking activity of the different immunization schemes was assessed using in vitro and in vivo assays. RESULTS: When Pbg37 and PSOP25 were co-administered in a mixture or as a fusion protein, they elicited similar antibody responses in mice as single antigens without causing immunological interference with each other. Antibodies against the mixed or fused antigens recognized the target proteins in the gametocyte, gamete, zygote, and ookinete stages. The mixed proteins or the fusion protein induced antibodies with significantly stronger transmission-reducing activities in vitro and in vivo than individual antigens. CONCLUSIONS: There was no immunological interference between Pbg37 and PSOP25. The bivalent vaccines, which expand the portion of the sexual development during which the transmission-blocking antibodies act, produced significantly stronger transmission-reducing activities than single antigens. Altogether, these data provide the theoretical basis for the development of combination TBVs targeting different sexual stages.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium berghei / Proteínas Protozoarias / Vacunas Combinadas / Vacunas contra la Malaria / Malaria Límite: Animals / Female / Humans Idioma: En Revista: Parasit Vectors Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium berghei / Proteínas Protozoarias / Vacunas Combinadas / Vacunas contra la Malaria / Malaria Límite: Animals / Female / Humans Idioma: En Revista: Parasit Vectors Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido