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Miniaturized probe on polymer SU-8 with array of individually addressable microelectrodes for electrochemical analysis in neural and other biological tissues.
Lotfi Marchoubeh, Mahsa; Cobb, Samuel J; Abrego Tello, Miguel; Hu, Mengjia; Jaquins-Gerstl, Andrea; Robbins, Elaine M; Macpherson, Julie V; Michael, Adrian C; Fritsch, Ingrid.
Afiliación
  • Lotfi Marchoubeh M; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
  • Cobb SJ; Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK.
  • Abrego Tello M; Department of Chemistry and Centre for Doctoral Training in Diamond Science and Technology, and Department of Physics, University of Warwick, Coventry, UK.
  • Hu M; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
  • Jaquins-Gerstl A; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
  • Robbins EM; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.
  • Macpherson JV; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.
  • Michael AC; Department of Chemistry and Centre for Doctoral Training in Diamond Science and Technology, and Department of Physics, University of Warwick, Coventry, UK.
  • Fritsch I; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.
Anal Bioanal Chem ; 413(27): 6777-6791, 2021 Nov.
Article en En | MEDLINE | ID: mdl-33961102
An SU-8 probe with an array of nine, individually addressable gold microband electrodes (100 µm long, 4 µm wide, separated by 4-µm gaps) was photolithographically fabricated and characterized for detection of low concentrations of chemicals in confined spaces and in vivo studies of biological tissues. The probe's shank (6 mm long, 100 µm wide, 100 µm thick) is flexible, but exhibits sufficient sharpness and rigidity to be inserted into soft tissue. Laser micromachining was used to define probe geometry by spatially revealing the underlying sacrificial aluminum layer, which was then etched to free the probes from a silicon wafer. Perfusion with fluorescent nanobeads showed that, like a carbon fiber electrode, the probe produced no noticeable damage when inserted into rat brain, in contrast to damage from an inserted microdialysis probe. The individual addressability of the electrodes allows single and multiple electrode activation. Redox cycling is possible, where adjacent electrodes serve as generators (that oxidize or reduce molecules) and collectors (that do the opposite) to amplify signals of small concentrations without background subtraction. Information about electrochemical mechanisms and kinetics may also be obtained. Detection limits for potassium ferricyanide in potassium chloride electrolyte of 2.19, 1.25, and 2.08 µM and for dopamine in artificial cerebral spinal fluid of 1.94, 1.08, and 5.66 µM for generators alone and for generators and collectors during redox cycling, respectively, were obtained.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Técnicas Electroquímicas / Microelectrodos Límite: Animals Idioma: En Revista: Anal Bioanal Chem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Técnicas Electroquímicas / Microelectrodos Límite: Animals Idioma: En Revista: Anal Bioanal Chem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania