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Crocin and Metformin suppress metastatic breast cancer progression via VEGF and MMP9 downregulations: in vitro and in vivo studies.
Farahi, Ali; Abedini, Mohammad Reza; Javdani, Hossein; Arzi, Laleh; Chamani, Elham; Farhoudi, Ramin; Talebloo, Nazanin; Hoshyar, Reyhane.
Afiliación
  • Farahi A; Student Research Committee and Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
  • Abedini MR; Cellular and Molecular Research Center, Birjand University of Medical Sciences, P.O. Box 9717853577, Birjand, Iran.
  • Javdani H; Cellular and Molecular Research Center, Birjand University of Medical Sciences, P.O. Box 9717853577, Birjand, Iran. mohrabe@yahoo.com.
  • Arzi L; Department of Cellular and Molecular Medicine, University of Ottawa School of Medicine, Ottawa, ON, Canada. mohrabe@yahoo.com.
  • Chamani E; Student Research Committee and Department of Molecular Medicine, Birjand University of Medical Sciences, Birjand, Iran.
  • Farhoudi R; Cellular and Molecular Research Center, Birjand University of Medical Sciences, P.O. Box 9717853577, Birjand, Iran.
  • Talebloo N; Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
  • Hoshyar R; Cellular and Molecular Research Center, Birjand University of Medical Sciences, P.O. Box 9717853577, Birjand, Iran.
Mol Cell Biochem ; 476(9): 3341-3351, 2021 Sep.
Article en En | MEDLINE | ID: mdl-33929675
Metastatic breast cancer remains a serious health concern and numerous investigations recommended medicinal plants as a complementary therapy. Crocin is one of the known anticancer bio-component. Recently, the inhibitory effect of metformin has been studied on the various aspects of cancer. However, no study reported their combination effects on metastatic breast cancer. In the present study, we have assessed their anti-metastatic effects on in vitro and in vivo breast cancer models. Using MTT assay, scratch, and adhesion tests, we have evaluated the cytotoxic, anti-invasive and anti-adhesion effects of crocin and metformin on 4T1 cell line, respectively. Their protective effects and MMP9 as well as VEGF protein expression levels (Western blotting) investigated in the 4T1 murine breast cancer model. Our results showed that both crocin and metformin reduced cell viability, delayed scratch healing and inhibited the cell adhesion, in vitro. While crocin alone restored the mice's weight reduction, crocin, metformin, and their combination significantly reduced the tumor volume size and enhanced animal survival rate in murine breast cancer model, responses that were associated with VEGF and MMP9 down-regulation. These findings suggest that a combination of crocin and metformin could serve as a novel therapeutic approach to enhance the effectiveness of metastatic breast cancer therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Carotenoides / Regulación Neoplásica de la Expresión Génica / Metaloproteinasa 9 de la Matriz / Factor A de Crecimiento Endotelial Vascular / Neoplasias Pulmonares / Metformina Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Mol Cell Biochem Año: 2021 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Carotenoides / Regulación Neoplásica de la Expresión Génica / Metaloproteinasa 9 de la Matriz / Factor A de Crecimiento Endotelial Vascular / Neoplasias Pulmonares / Metformina Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Mol Cell Biochem Año: 2021 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos