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First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia.
Lachkhem, Arwa; Galal, Lokman; Lahmar, Ibtissem; Passebosc, Karine; Riahi, Homayoun; Plault, Nicolas; Dardé, Marie-Laure; Mercier, Aurélien; Babba, Hamouda.
Afiliación
  • Lachkhem A; Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire (Code LR12ES08), Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir, Tunisia. lachkemarwa@gmail.com.
  • Galal L; INSERM, Université Limoges, CHU Limoges, IRD, U1094 Neuroépidémiologie Tropicale, Institut d'Epidémiologie et de Neurologie Tropicale, GEIST, Limoges, France.
  • Lahmar I; Laboratoire de Parasitologie-Mycologie Médicale et Moléculaire (Code LR12ES08), Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Monastir, Tunisia. ibtissem.lahmar75@gmail.com.
  • Passebosc K; Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Center (BRC), Centre Hospitalier-Universitaire Dupuytren, Limoges, France.
  • Riahi H; Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Center (BRC), Centre Hospitalier-Universitaire Dupuytren, Limoges, France.
  • Plault N; INSERM, Université Limoges, CHU Limoges, IRD, U1094 Neuroépidémiologie Tropicale, Institut d'Epidémiologie et de Neurologie Tropicale, GEIST, Limoges, France.
  • Dardé ML; INSERM, Université Limoges, CHU Limoges, IRD, U1094 Neuroépidémiologie Tropicale, Institut d'Epidémiologie et de Neurologie Tropicale, GEIST, Limoges, France.
  • Mercier A; Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Center (BRC), Centre Hospitalier-Universitaire Dupuytren, Limoges, France.
  • Babba H; INSERM, Université Limoges, CHU Limoges, IRD, U1094 Neuroépidémiologie Tropicale, Institut d'Epidémiologie et de Neurologie Tropicale, GEIST, Limoges, France.
Sci Rep ; 11(1): 9328, 2021 04 29.
Article en En | MEDLINE | ID: mdl-33927299
The isolation and molecular typing of Toxoplasma gondii strains provide an essential basis for a better understanding of the parasite's genetic diversity, determinants of its geographical distribution and associated risks to human health. In this study, we isolated and genetically characterized T. gondii strains from domestic animals in Southern and coastal area of Tunisia. Blood, hearts and/or brains were collected from 766 domestic animals (630 sheep and 136 free-range chickens). Strain isolation from these samples was performed using mouse bioassay and genotyping was carried out with a multiplex PCR technique using 15 microsatellite markers. Thirty viable strains of T. gondii were successfully isolated from tissues of sheep (19/142) and chickens (11/33). In addition, 3 strains could be successfully genotyped from animal tissues for which mouse bioassay was unsuccessful. A large predominance of type II strains (n = 29) was found in the sampled regions, followed by type III (n = 3) and, for the first time in Tunisia, a single isolate of Africa 4 lineage from a sheep. Analyses of population genetics showed the presence of a divergent population of type II lineage in Tunisia, supporting limited recent migrations of strains between Tunisia and other countries of the world.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Ovinos / Pollos Límite: Animals País/Región como asunto: Africa Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Ovinos / Pollos Límite: Animals País/Región como asunto: Africa Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Reino Unido