Knockdown of the Ribosomal Protein eL38 in HEK293 Cells Changes the Translational Efficiency of Specific Genes.

Gopanenko, Alexander V; Kolobova, Alena V; Tupikin, Alexey E; Kabilov, Marsel R; Malygin, Alexey A; Karpova, Galina G

Gopanenko, Alexander V; Kolobova, Alena V; Tupikin, Alexey E; Kabilov, Marsel R; Malygin, Alexey A; Karpova, Galina G.

Afiliación

Gopanenko AV; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, 630090 Novosibirsk, Russia.
Kolobova AV; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, 630090 Novosibirsk, Russia.
Tupikin AE; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, 630090 Novosibirsk, Russia.
Kabilov MR; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, 630090 Novosibirsk, Russia.
Malygin AA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, 630090 Novosibirsk, Russia.
Karpova GG; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, 630090 Novosibirsk, Russia.

Int J Mol Sci ; 22(9)2021 Apr 26.

Article en En | MEDLINE | ID: mdl-33926116

RESUMEN

The protein eL38 is one of the smallest proteins of the mammalian ribosome, which is a component of its large (60S) subunit. The haploinsufficiency of eL38 in mice leads to the Tail-short mutant phenotype characterized by defects in the development of the axial skeleton caused by the poor translation of mRNA subsets of Hox genes. Using the ribosome profiling assay applied to HEK293 cells knocked down of eL38, we examined the effects of the lack of eL38 in 60S subunits on gene expression at the level of translation. A four-fold decrease in the cell content of eL38 was shown to result in significant changes in the translational efficiencies of 150 genes. Among the genes, whose expression at the level of translation was enhanced, there were mainly those associated with basic metabolic processes; namely, translation, protein folding, chromosome organization, splicing, and others. The set of genes with reduced translation efficiencies contained those that are mostly involved in the processes related to the regulation of transcription, including the activation of Hox genes. Thus, we demonstrated that eL38 insufficiency significantly affects the expression of certain genes at the translational level. Our findings facilitate understanding the possible causes of some anomalies in eL38-deficient animals.

Asunto(s)

Regulación de la Expresión Génica/genética; Proteínas Ribosómicas/genética; Proteínas Ribosómicas/metabolismo; Expresión Génica/genética; Células HEK293; Humanos; Biosíntesis de Proteínas; ARN Mensajero/genética; Subunidades Ribosómicas Grandes de Eucariotas/genética; Subunidades Ribosómicas Grandes de Eucariotas/metabolismo; Ribosomas/metabolismo; Transcriptoma/genética

Palabras clave

HEK293 cells; Ribo-seq; eL38-related processes; genes with eL38-dependent translational efficiencies; knockdown of ribosomal protein eL38; next-generation sequencing

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Ribosómicas / Regulación de la Expresión Génica Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza

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