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The Role of Galectin-9 as Mediator of Atopic Dermatitis: Effect on Keratinocytes.
Corrêa, Mab P; Areias, Libnah L; Correia-Silva, Rebeca D; D'Ávila, Solange C G P; Leopoldino, Andréia M; Greco, Karin V; Gil, Cristiane D.
Afiliación
  • Corrêa MP; Programa de Pós-Graduação em Biociências, Instituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
  • Areias LL; Departamento de Morfologia e Genética, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP 04023-900, Brazil.
  • Correia-Silva RD; Departamento de Morfologia e Genética, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP 04023-900, Brazil.
  • D'Ávila SCGP; Departamento de Patologia e Medicina Forense, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP 15090-000, Brazil.
  • Leopoldino AM; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP 14040-903, Brazil.
  • Greco KV; Division of Surgery and Interventional Science, The Griffin Institute, University College London (UCL), London HA13UJ, UK.
  • Gil CD; Programa de Pós-Graduação em Biociências, Instituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista (UNESP), São José do Rio Preto, SP 15054-000, Brazil.
Cells ; 10(4)2021 04 20.
Article en En | MEDLINE | ID: mdl-33923930
Galectin-9 (Gal-9) is a beta-galactoside-binding protein with a variety of biological functions related to immune response. However, in allergic diseases, its mechanism of action is not fully understood. This study evaluates the expression pattern of Gal-9 in patients with atopic dermatitis (AD), in ovalbumin (OVA)-induced experimental atopic dermatitis (AD) in mice, as well as its effect on human keratinocytes. The skin of OVA-immunized BALB/c mice was challenged with drops containing OVA on days 11, 14-18, and 21-24. HaCaT cells were cultured in the following experimental conditions: control (growth medium only) or stimulated with TNF-α/IFN-γ, or IL-4, or IL-17 with or without Gal-9 treatment. AD was characterized by increased levels of Gal-9 in mouse and human skin, especially in the epidermis, and with a marked influx of Gal-9 positive eosinophils and mast cells compared to the control group. Gal-9 showed an immunomodulatory effect on keratinocytes by decreasing the release of IL-6 by IL-4-stimulated keratinocytes or increasing the IL-6 and RANTES levels by IL-17- or TNF-α/IFN-γ-stimulated cells, respectively. Under IL-17, Gal-9 treatment also altered the proliferation rate of cells. Overall, increased levels of Gal-9 in AD skin contribute to the control of inflammatory response and the proliferative process of keratinocytes, suggesting this lectin as a relevant therapeutic target.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Galectinas / Dermatitis Atópica Límite: Animals / Humans / Male Idioma: En Revista: Cells Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Galectinas / Dermatitis Atópica Límite: Animals / Humans / Male Idioma: En Revista: Cells Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza