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Characteristics of Anti-SARS-CoV-2 Antibodies in Recovered COVID-19 Subjects.
Huynh, Angela; Arnold, Donald M; Smith, James W; Moore, Jane C; Zhang, Ali; Chagla, Zain; Harvey, Bart J; Stacey, Hannah D; Ang, Jann C; Clare, Rumi; Ivetic, Nikola; Chetty, Vasudhevan T; Bowdish, Dawn M E; Miller, Matthew S; Kelton, John G; Nazy, Ishac.
Afiliación
  • Huynh A; Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.
  • Arnold DM; Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.
  • Smith JW; McMaster Centre for Transfusion Research, McMaster University, Hamilton, ON L8N 3Z5, Canada.
  • Moore JC; Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.
  • Zhang A; Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.
  • Chagla Z; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.
  • Harvey BJ; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.
  • Stacey HD; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada.
  • Ang JC; Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.
  • Clare R; Division of Infectious Diseases, Department of Medicine, McMaster University, Hamilton, ON L8V 1C3, Canada.
  • Ivetic N; Hamilton Public Health Services, Hamilton, ON L8P 4Y5, Canada.
  • Chetty VT; Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada.
  • Bowdish DME; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.
  • Miller MS; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.
  • Kelton JG; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada.
  • Nazy I; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.
Viruses ; 13(4)2021 04 16.
Article en En | MEDLINE | ID: mdl-33923828
Coronavirus Disease 2019 (COVID-19) is a global pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While detection of SARS-CoV-2 by polymerase chain reaction with reverse transcription (RT-PCR) is currently used to diagnose acute COVID-19 infection, serological assays are needed to study the humoral immune response to SARS-CoV-2. Anti-SARS-CoV-2 immunoglobulin (Ig)G/A/M antibodies against spike (S) protein and its receptor-binding domain (RBD) were characterized in recovered subjects who were RT-PCR-positive (n = 153) and RT-PCR-negative (n = 55) using an enzyme-linked immunosorbent assay (ELISA). These antibodies were also further assessed for their ability to neutralize live SARS-CoV-2 virus. Anti-SARS-CoV-2 antibodies were detected in 90.9% of resolved subjects up to 180 days post-symptom onset. Anti-S protein and anti-RBD IgG titers correlated (r = 0.5157 and r = 0.6010, respectively) with viral neutralization. Of the RT-PCR-positive subjects, 22 (14.3%) did not have anti-SARS-CoV-2 antibodies; and of those, 17 had RT-PCR cycle threshold (Ct) values > 27. These high Ct values raise the possibility that these indeterminate results are from individuals who were not infected or had mild infection that failed to elicit an antibody response. This study highlights the importance of serological surveys to determine population-level immunity based on infection numbers as determined by RT-PCR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza