hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination.

Evtushenko, Nadezhda A; Beilin, Arkadii K; Dashinimaev, Erdem B; Ziganshin, Rustam H; Kosykh, Anastasiya V; Perfilov, Maxim M; Rippa, Alexandra L; Alpeeva, Elena V; Vasiliev, Andrey V; Vorotelyak, Ekaterina A; Gurskaya, Nadya G

Evtushenko, Nadezhda A; Beilin, Arkadii K; Dashinimaev, Erdem B; Ziganshin, Rustam H; Kosykh, Anastasiya V; Perfilov, Maxim M; Rippa, Alexandra L; Alpeeva, Elena V; Vasiliev, Andrey V; Vorotelyak, Ekaterina A; Gurskaya, Nadya G.

Afiliación

Evtushenko NA; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, Russia.
Beilin AK; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, Russia.
Dashinimaev EB; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilova Str., 119334 Moscow, Russia.
Ziganshin RH; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, Russia.
Kosykh AV; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilova Str., 119334 Moscow, Russia.
Perfilov MM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Rippa AL; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, Ostrovityanova 1, 117997 Moscow, Russia.
Alpeeva EV; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilova Str., 119334 Moscow, Russia.
Vasiliev AV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Vorotelyak EA; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilova Str., 119334 Moscow, Russia.
Gurskaya NG; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilova Str., 119334 Moscow, Russia.

Int J Mol Sci ; 22(8)2021 Apr 07.

Article en En | MEDLINE | ID: mdl-33916959

Asunto(s)

Fibroblastos/metabolismo; Recombinación Homóloga; Integrasas/metabolismo; Queratinocitos/metabolismo; Telomerasa/genética; Transgenes; Adolescente; Adulto; Biomarcadores; Línea Celular Transformada; Proliferación Celular; Senescencia Celular/genética; Niño; Epidermólisis Ampollosa Distrófica/etiología; Epidermólisis Ampollosa Distrófica/metabolismo; Femenino; Fibroblastos/patología; Técnica del Anticuerpo Fluorescente; Técnicas de Silenciamiento del Gen; Orden Génico; Vectores Genéticos/genética; Humanos; Inmunohistoquímica; Masculino; Persona de Mediana Edad; Mutación; Complejo Represivo Polycomb 1/genética; Complejo Represivo Polycomb 1/metabolismo; Cultivo Primario de Células; Proteómica/métodos; Telomerasa/metabolismo; Adulto Joven

Palabras clave

3D skin equivalent; COL7A1; Cre recombination; RDEB; bmi-1; fibroblast; hTERT; immortalization; keratinocyte; secretomes; stratification

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Transgenes / Telomerasa / Integrasas / Recombinación Homóloga / Fibroblastos Tipo de estudio: Etiology_studies Límite: Adolescent / Adult / Child / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza

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