[Insulin-like growth factor-1 alleviates hepatocyte senescence by regulating intranuclear p53-progerin pathway].

Jiang, X K; Li, J; Bai, Y Q; Ding, H; Yang, Z Y; Sun, S F; Liang, Y; Peng, C; Han, S Y; Li, X L; Luo, X Y; Zhang, B Y

Jiang, X K; Li, J; Bai, Y Q; Ding, H; Yang, Z Y; Sun, S F; Liang, Y; Peng, C; Han, S Y; Li, X L; Luo, X Y; Zhang, B Y.

Afiliación

Jiang XK; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Li J; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Bai YQ; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Ding H; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Yang ZY; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Sun SF; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Liang Y; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Peng C; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Han SY; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Li XL; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Luo XY; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
Zhang BY; Department of Gastroenterology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.

Zhonghua Gan Zang Bing Za Zhi ; 29(3): 271-274, 2021 Mar 20.

Article en Zh | MEDLINE | ID: mdl-33902196

RESUMEN

To construct cellular senescence model by stimulating primary hepatocytes with hydrogen peroxide (H(2)O(2)). Primary hepatocytes were transfected with p53 siRNA, progerin siRNA or IGF-1 adenovirus vector. The number of SA-ß-Gal stained positive cells and the expression of p53 and progerin were detected. The results showed that p53 siRNA and progerin siRNA had knocked-down the expression of p53 and progerin, and had alleviated the hepatocyte senescence. Transfection of insulin-like growth factor (IGF)-1 adenovirus vector into primary hepatocytes had overexpressed IGF-1, and had alleviated the number of SA-ß-Gal-positive cells. The expression of p53 and progerin was down-regulated in the nucleus, while the expression of p53 was up-regulated in the cytoplasm. The co-precipitation and co-localization of p53 and progerin was decreased in the nuclear region of hepatocytes. IGF-1 overexpression can inhibit intranuclear p53 translocation, alleviate the interaction between p53-progerin, and alleviate hepatocyte senescence.

Asunto(s)

Factor I del Crecimiento Similar a la Insulina; Proteína p53 Supresora de Tumor; Senescencia Celular; Hepatocitos; Peróxido de Hidrógeno; Proteína p53 Supresora de Tumor/genética

Palabras clave

Hepatocyte senescence; Insulin-like growth factor; Progerin; p53

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor I del Crecimiento Similar a la Insulina / Proteína p53 Supresora de Tumor Tipo de estudio: Prognostic_studies Idioma: Zh Revista: Zhonghua Gan Zang Bing Za Zhi Asunto de la revista: GASTROENTEROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: China

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