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Production of D-lactic acid containing polyhydroxyalkanoate polymers in yeast Saccharomyces cerevisiae.
Ylinen, Anna; Maaheimo, Hannu; Anghelescu-Hakala, Adina; Penttilä, Merja; Salusjärvi, Laura; Toivari, Mervi.
Afiliación
  • Ylinen A; VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
  • Maaheimo H; VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
  • Anghelescu-Hakala A; VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
  • Penttilä M; VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
  • Salusjärvi L; Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, P.O. Box 11000, FI-00076 Aalto, Espoo, Finland.
  • Toivari M; VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland.
J Ind Microbiol Biotechnol ; 48(5-6)2021 Jul 01.
Article en En | MEDLINE | ID: mdl-33899921
Polyhydroxyalkanoates (PHAs) provide biodegradable and bio-based alternatives to conventional plastics. Incorporation of 2-hydroxy acid monomers into polymer, in addition to 3-hydroxy acids, offers possibility to tailor the polymer properties. In this study, poly(D-lactic acid) (PDLA) and copolymer P(LA-3HB) were produced and characterized for the first time in the yeast Saccharomyces cerevisiae. Expression of engineered PHA synthase PhaC1437Ps6-19, propionyl-CoA transferase Pct540Cp, acetyl-CoA acetyltransferase PhaA, and acetoacetyl-CoA reductase PhaB1 resulted in accumulation of 3.6% P(LA-3HB) and expression of engineered enzymes PhaC1Pre and PctMe resulted in accumulation of 0.73% PDLA of the cell dry weight (CDW). According to NMR, P(LA-3HB) contained D-lactic acid repeating sequences. For reference, expression of PhaA, PhaB1, and PHA synthase PhaC1 resulted in accumulation 11% poly(hydroxybutyrate) (PHB) of the CDW. Weight average molecular weights of these polymers were comparable to similar polymers produced by bacterial strains, 24.6, 6.3, and 1 130 kDa for P(LA-3HB), PDLA, and PHB, respectively. The results suggest that yeast, as a robust and acid tolerant industrial production organism, could be suitable for production of 2-hydroxy acid containing PHAs from sugars or from 2-hydroxy acid containing raw materials. Moreover, the wide substrate specificity of PHA synthase enzymes employed increases the possibilities for modifying copolymer properties in yeast in the future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Ácido Láctico / Polihidroxialcanoatos Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Ácido Láctico / Polihidroxialcanoatos Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Alemania