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Genome characterization of parsley severe stunt-associated virus in Iran.
Hasanvand, Vahid; Heydanejad, Jahangir; Massumi, Hossain; Kleinow, Tatjana; Jeske, Holger; Fontenele, Rafaela S; Kraberger, Simona; Varsani, Arvind.
Afiliación
  • Hasanvand V; Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111, Kerman, Iran.
  • Heydanejad J; Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111, Kerman, Iran. jheydarnejad@uk.ac.ir.
  • Massumi H; Research and Technology Institute of Plant Production (RTIPP), Shahid Bahonar University of Kerman, 7616914111, Kerman, Iran. jheydarnejad@uk.ac.ir.
  • Kleinow T; Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, 7616914111, Kerman, Iran.
  • Jeske H; Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany.
  • Fontenele RS; Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany.
  • Kraberger S; The Biodesign Center of Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and Medicine, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ, 85287-5001, USA.
  • Varsani A; The Biodesign Center of Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and Medicine, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ, 85287-5001, USA.
Virus Genes ; 57(3): 293-301, 2021 Jun.
Article en En | MEDLINE | ID: mdl-33881682
Parsley severe stunt-associated virus (PSSaV) is a recently identified nanovirus first reported in Germany. During a survey for identification of nanoviruses infecting apiaceous plants in south-eastern Iran, PSSaV was identified and characterized using a combination of rolling circle amplification (RCA) and high-throughput sequencing. Parsley plant samples were collected from vegetable production farms in Kerman province. From two symptomatic samples (39Ba and 40Ba), seven PSSaV components (DNA-C, -S, -M, -R, -N, -U1 and -U2) with two phylogenetically distinct variants of DNA-R (R1 and R2) were identified. In common with the German isolate of PSSaV, no DNA-U4 component was identified. In addition, associated alphasatellite molecules were identified in samples 39Ba [n = 6] and 40Ba [n = 5]. Sequence analyses showed that concatenated component sequences of the two Iranian PSSaVs share 97.2% nucleotide identity with each other and 82% to the German isolate. The coat proteins (CPs) of the PSSaV Iranian sequences share 97.2% amino acid identity and ~ 84% identity with that of the German isolate. Sequence and phylogenetic analyses of a total of 11 recovered alphasatellites from the two samples can be classified into the genera Fabenesatellite [n = 2], Milvetsatellite [n = 1], Mivedwarsatellite [n = 2], Subclovsatellite [n = 2], Sophoyesatellite [n = 4] in the family Alphasatellitidae. Identification of PSSaV and other nanoviruses in wild and cultivated plants in Iran reveals that nanoviruses could be causing yield reduction in crops plants in this country.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Virus de Plantas / Genoma Viral / Petroselinum Tipo de estudio: Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Virus Genes Asunto de la revista: BIOLOGIA MOLECULAR / VIROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Virus de Plantas / Genoma Viral / Petroselinum Tipo de estudio: Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Virus Genes Asunto de la revista: BIOLOGIA MOLECULAR / VIROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos