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Platelet-rich plasma enhances mechanical strength of strattice in rat model of ventral hernia repair.
Fernandez-Moure, Joseph S; Van Eps, Jeffrey L; Scherba, Jacob C; Yazdi, Iman K; Robbins, Andrew; Cabrera, Fernando; Vatsaas, Cory; Moreno, Michael; Weiner, Bradley K; Tasciotti, Ennio.
Afiliación
  • Fernandez-Moure JS; Division of Trauma, Acute, and Critical Care Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA.
  • Van Eps JL; University of Texas Health Science Center, McGovern Medical School, Houston, Texas, USA.
  • Scherba JC; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
  • Yazdi IK; Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA.
  • Robbins A; Department of Medicine, Renal Division, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Cabrera F; Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA.
  • Vatsaas C; Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA.
  • Moreno M; Division of Trauma, Acute, and Critical Care Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA.
  • Weiner BK; Department of Mechanical Engineering, Texas A&M College of Medicine, Bryan, Texas, USA.
  • Tasciotti E; Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA.
J Tissue Eng Regen Med ; 15(7): 634-647, 2021 07.
Article en En | MEDLINE | ID: mdl-33880858
Incisional hernia is a common complication of hernia repair despite the development of various synthetic and bio-synthetic repair materials. Poor long-term mechanical strength, leading to high recurrence rates, has limited the use of acellular dermal matrices (ADMs) in ventral hernia repair (VHR). Biologically derived meshes have been an area of increasing interest. Still these materials bring the risk of more aggressive immune response and fibrosis in addition to the mechanical failures suffered by the synthetic materials. Platelet-rich plasma (PRP), a growth-factor-rich autologous blood product, has been shown to improve early neovascularization, tissue deposition, and to decrease the rates of recurrence. Here, we demonstrate that PRP promotes the release of growth factors stromal derived factor (SDF)-1, transforming growth factor-beta, and platelet-derived growth factor in a dose-dependent manner. Additionally, we utilize an aortic ring angiogenesis assay to show that PRP promotes angiogenesis in vitro. A rat model of VHR using StratticeTM ADM demonstrates similar findings in vivo, corresponding with the increased expression of vascular endothelial growth factor and collagen type 1 alpha 1. Finally, we show that the molecular and cellular activity initiated by PRP results in an increased mechanical stiffness of the hernia repair mesh over time. Collectively, these data represent an essential step in demonstrating the utility and the mechanism of platelet-derived plasma in biomaterial-aided wound healing and provide promising preclinical data that suggest such materials may improve surgical outcomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma Rico en Plaquetas / Herniorrafia / Hernia Ventral Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Tissue Eng Regen Med Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma Rico en Plaquetas / Herniorrafia / Hernia Ventral Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Tissue Eng Regen Med Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido