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Mass spectrometry-based proteomic platforms for better understanding of SARS-CoV-2 induced pathogenesis and potential diagnostic approaches.
Ahsan, Nagib; Rao, R Shyama Prasad; Wilson, Rashaun S; Punyamurtula, Ujwal; Salvato, Fernanda; Petersen, Max; Ahmed, Mohammad Kabir; Abid, M Ruhul; Verburgt, Jacob C; Kihara, Daisuke; Yang, Zhibo; Fornelli, Luca; Foster, Steven B; Ramratnam, Bharat.
Afiliación
  • Ahsan N; Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA.
  • Rao RSP; Biostatistics and Bioinformatics Division, Yenepoya Research Center, Yenepoya University, Mangaluru, India.
  • Wilson RS; Keck Mass Spectrometry and Proteomics Resource, Yale University, New Haven, Connecticut, USA.
  • Punyamurtula U; COBRE Center for Cancer Research Development, Proteomics Core Facility, Rhode Island Hospital, Providence, Rhode Island, USA.
  • Salvato F; Department of Plant and Microbial Biology, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, North Carolina, USA.
  • Petersen M; Signal Transduction Lab, Division of Hematology/Oncology, Rhode Island Hospital, Warren Alpert Medical School, Brown University, Providence, Rhode Island, USA.
  • Ahmed MK; Department of Biochemistry, Faculty of Medicine, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh, Perak, Malaysia.
  • Abid MR; Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Warren Alpert Medical School, Brown University, Providence, Rhode Island, USA.
  • Verburgt JC; Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA.
  • Kihara D; Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA.
  • Yang Z; Department of Computer Science, Purdue University, West Lafayette, Indiana, USA.
  • Fornelli L; Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA.
  • Foster SB; Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA.
  • Ramratnam B; Department of Biology, University of Oklahoma, Norman, Oklahoma, USA.
Proteomics ; 21(10): e2000279, 2021 05.
Article en En | MEDLINE | ID: mdl-33860983
While protein-protein interaction is the first step of the SARS-CoV-2 infection, recent comparative proteomic profiling enabled the identification of over 11,000 protein dynamics, thus providing a comprehensive reflection of the molecular mechanisms underlying the cellular system in response to viral infection. Here we summarize and rationalize the results obtained by various mass spectrometry (MS)-based proteomic approaches applied to the functional characterization of proteins and pathways associated with SARS-CoV-2-mediated infections in humans. Comparative analysis of cell-lines versus tissue samples indicates that our knowledge in proteome profile alternation in response to SARS-CoV-2 infection is still incomplete and the tissue-specific response to SARS-CoV-2 infection can probably not be recapitulated efficiently by in vitro experiments. However, regardless of the viral infection period, sample types, and experimental strategies, a thorough cross-comparison of the recently published proteome, phosphoproteome, and interactome datasets led to the identification of a common set of proteins and kinases associated with PI3K-Akt, EGFR, MAPK, Rap1, and AMPK signaling pathways. Ephrin receptor A2 (EPHA2) was identified by 11 studies including all proteomic platforms, suggesting it as a potential future target for SARS-CoV-2 infection mechanisms and the development of new therapeutic strategies. We further discuss the potentials of future proteomics strategies for identifying prognostic SARS-CoV-2 responsive age-, gender-dependent, tissue-specific protein targets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Proteómica / Interacciones Huésped-Patógeno / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Proteómica / Interacciones Huésped-Patógeno / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania