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STAT6-Dependent Exacerbation of House Dust Mite-Induced Allergic Airway Disease in Mice by Multi-Walled Carbon Nanotubes.
Ihrie, Mark D; Duke, Katherine S; Shipkowski, Kelly A; You, Dorothy J; Lee, Ho Young; Taylor-Just, Alexia J; Bonner, James C.
Afiliación
  • Ihrie MD; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
  • Duke KS; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
  • Shipkowski KA; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
  • You DJ; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
  • Lee HY; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
  • Taylor-Just AJ; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
  • Bonner JC; Toxicology Program, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695 U.S.A.
NanoImpact ; 222021 04.
Article en En | MEDLINE | ID: mdl-33860111
There is increasing evidence that inhaled multi-walled carbon nanotubes (MWCNTs) can have harmful effects on the respiratory system. Rodent studies suggest that individuals with asthma may be susceptible to the adverse pulmonary effects of MWCNTs. Asthma is an allergic lung disease characterized by a TH2 immune response that results in chronic airway disease characterized by eosinophilic lung inflammation, airway mucous cell metaplasia, and airway fibrosis. Signal transducer and activator of transcription 6 (STAT6) is a transcription factor with multiple roles in TH2 type inflammation. Herein we sought to examine the role of STAT6 in the exacerbation of house dust mite (HDM) allergen-induced allergic airway disease by MWCNTs. Male wild type (WT) and STAT6 knockout (Stat6 KO) mice were dosed via intranasal aspiration on days 0, 2, 4, 14, 16 and 18 with either vehicle, HDM extract, MWCNTs, or a combination of HDM and MWCNTs. Necropsy was performed on day 21 to collect bronchoalveolar lavage fluid (BALF), serum and lung tissue. MWCNTs exacerbated HDM-induced allergic endpoints, including eosinophilic lung inflammation, mucous cell metaplasia, and serum IgE levels. HDM-induced eosinophilic lung inflammation, mucous cell metaplasia, and serum IgE and exacerbation of these endpoints by MWCNTs were ablated in Stat6 KO mice. In addition, airway fibrosis was significantly increased by the combination of HDM and MWCNTs in WT mice but not in Stat6 KO mice. These findings provide new mechanistic insight by demonstrating a requirement for STAT6 in MWCNT-induced exacerbation of allergic respiratory disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pyroglyphidae / Nanotubos de Carbono Límite: Animals Idioma: En Revista: NanoImpact Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pyroglyphidae / Nanotubos de Carbono Límite: Animals Idioma: En Revista: NanoImpact Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos