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Helicobacter Pylori Variants with ABC-Type Tyrosine Phosphorylation Motif in Gastric Biopsies of Ghanaian Patients.
Tagoe, Emmanuel A; Awandare, Gordon A; Quaye, Osbourne; Asmah, Richard H; Archampong, Timothy N; Osman, Mahasin A; Brown, Charles A.
Afiliación
  • Tagoe EA; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP)/Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Accra, Ghana.
  • Awandare GA; Department of Medical Laboratory Sciences, University of Ghana, Korle Bu, Accra, Ghana.
  • Quaye O; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP)/Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Accra, Ghana.
  • Asmah RH; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP)/Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Accra, Ghana.
  • Archampong TN; Department of Medical Laboratory Sciences, University of Ghana, Korle Bu, Accra, Ghana.
  • Osman MA; Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Allied Health Sciences, Ho, Ghana.
  • Brown CA; Department of Medicine, University of Ghana Medical School, University of Ghana, Korle Bu, Accra, Ghana.
Biomed Res Int ; 2021: 6616059, 2021.
Article en En | MEDLINE | ID: mdl-33860041
BACKGROUND: Helicobacter pylori pathogenicity and disease severity are determined by the tyrosine phosphorylation motifs of CagA protein. This study is aimed at detecting the presence of H. pylori and identifying the CagA tyrosine phosphorylation motifs in Ghanaian patients. Material and Methods. A total of 94 archival genomic DNA samples from gastric biopsies were used for the study, and H. pylori was detected by amplifying the 16S rRNA gene. The 3'-end variable region of the cagA gene was amplified, and the entire 3'-end was sequenced and translated into amino acids. RESULTS: H. pylori was detected in 53.2% (50/94) of the samples, and all the detected bacteria harboured the cagA gene. Two variants of the bacteria were identified based on the size of the amplified cagA gene: 207 bp and 285 bp. The 207 bp and 285 bp variants accounted for 74% and 22%, respectively, and 4% showed both fragments. Translated amino acid sequence of the cagA gene showed EPIYA-A, EPIYA-B, and EPIYA-C (ABC type) motifs, indicating the Western variant. The CagA protein C-terminal showed insertion of amino acids in the sequence flanking the EPIYA-A motif at the N-terminal and a complete deletion of the EPIYA-CC and EPIYA-CCC motifs together with the flanking sequences. CONCLUSIONS: H. pylori identified were Western variant (ABC type) with unique amino acid insertions, suggesting unique variants in Ghanaian patients. Further investigation is however required to understand the role of the molecular diversity of the variant in gastric disease outcome.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estómago / Tirosina / Proteínas Bacterianas / Helicobacter pylori / Antígenos Bacterianos Tipo de estudio: Prognostic_studies Límite: Humans País/Región como asunto: Africa Idioma: En Revista: Biomed Res Int Año: 2021 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estómago / Tirosina / Proteínas Bacterianas / Helicobacter pylori / Antígenos Bacterianos Tipo de estudio: Prognostic_studies Límite: Humans País/Región como asunto: Africa Idioma: En Revista: Biomed Res Int Año: 2021 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Estados Unidos