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Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14.
Mahjoubi, Mouna; Aliyu, Habibu; Neifar, Mohamed; Cappello, Simone; Chouchane, Habib; Souissi, Yasmine; Masmoudi, Ahmed Salaheddine; Cowan, Don A; Cherif, Ameur.
Afiliación
  • Mahjoubi M; University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole SidiThabet, 2020, Ariana, Tunisia.
  • Aliyu H; Institute of Process Engineering in Life Science 2: Technical Biology, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Neifar M; University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole SidiThabet, 2020, Ariana, Tunisia.
  • Cappello S; Istituto per le Risorse Biologiche e le Biotecnologie Marine (IRBIM)-CNR of Messina, Sp. San Raineri, 86, 98122, Messina, Italy.
  • Chouchane H; University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole SidiThabet, 2020, Ariana, Tunisia.
  • Souissi Y; University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole SidiThabet, 2020, Ariana, Tunisia.
  • Masmoudi AS; University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole SidiThabet, 2020, Ariana, Tunisia.
  • Cowan DA; Centre for Microbial Ecology and Genomics, University of Pretoria, Pretoria, 0002, South Africa.
  • Cherif A; University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole SidiThabet, 2020, Ariana, Tunisia. cherif.ameur@gmail.com.
Sci Rep ; 11(1): 8124, 2021 04 14.
Article en En | MEDLINE | ID: mdl-33854112
Bioremediation offers a viable alternative for the reduction of contaminants from the environment, particularly petroleum and its recalcitrant derivatives. In this study, the ability of a strain of Pseudomonas BUN14 to degrade crude oil, pristane and dioxin compounds, and to produce biosurfactants, was investigated. BUN14 is a halotolerant strain isolated from polluted sediment recovered from the refinery harbor on the Bizerte coast, north Tunisia and capable of producing surfactants. The strain BUN14 was assembled into 22 contigs of 4,898,053 bp with a mean GC content of 62.4%. Whole genome phylogeny and comparative genome analyses showed that strain BUN14 could be affiliated with two validly described Pseudomonas Type Strains, P. kunmingensis DSM 25974T and P. chloritidismutans AW-1T. The current study, however, revealed that the two Type Strains are probably conspecific and, given the priority of the latter, we proposed that P. kunmingensis DSM 25974 is a heteronym of P. chloritidismutans AW-1T. Using GC-FID analysis, we determined that BUN14 was able to use a range of hydrocarbons (crude oil, pristane, dibenzofuran, dibenzothiophene, naphthalene) as a sole carbon source. Genome analysis of BUN14 revealed the presence of a large repertoire of proteins (154) related to xenobiotic biodegradation and metabolism. Thus, 44 proteins were linked to the pathways for complete degradation of benzoate and naphthalene. The annotation of conserved functional domains led to the detection of putative genes encoding enzymes of the rhamnolipid biosynthesis pathway. Overall, the polyvalent hydrocarbon degradation capacity of BUN14 makes it a promising candidate for application in the bioremediation of polluted saline environments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas / Genoma Bacteriano País/Región como asunto: Africa Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas / Genoma Bacteriano País/Región como asunto: Africa Idioma: En Revista: Sci Rep Año: 2021 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Reino Unido