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A recombinant Chinese measles virus vaccine strain rMV-Hu191 inhibits human colorectal cancer growth through inducing autophagy and apoptosis regulating by PI3K/AKT pathway.
Zhang, Chu-di; Wang, Yi-Long; Zhou, Dong-Ming; Zhu, Meng-Ying; Lv, Yao; Hao, Xiao-Qiang; Qu, Chu-Fan; Chen, Yi; Gu, Wei-Zhong; Wu, Ben-Qing; Chen, Pei-Chun; Zhao, Zheng-Yan.
Afiliación
  • Zhang CD; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China; Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang. China. Electronic address: 21718442@zju.edu.cn.
  • Wang YL; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China. Electronic address: 11418155@zju.edu.cn.
  • Zhou DM; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China. Electronic address: 21318243@zju.edu.cn.
  • Zhu MY; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China; Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang. China. Electronic address: 11618182@zju.edu.cn.
  • Lv Y; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China. Electronic address: 21418068@zju.edu.cn.
  • Hao XQ; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China. Electronic address: 11718196@zju.edu.cn.
  • Qu CF; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China; Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang. China. Electronic address: 21818469@zju.edu.cn.
  • Chen Y; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China; Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang. China. Electronic address: 21918473@zju.edu.cn.
  • Gu WZ; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China. Electronic address: 6195013@zju.edu.cn.
  • Wu BQ; University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518000, China. Electronic address: wubenqing783@126.com.
  • Chen PC; University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen 518000, China. Electronic address: 674870541@qq.com.
  • Zhao ZY; Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, Zhejiang. China; Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang. China. Electronic address: zhaozy@zju.edu.cn.
Transl Oncol ; 14(7): 101091, 2021 Jul.
Article en En | MEDLINE | ID: mdl-33848808
The potential therapeutic effects of oncolytic measles virotherapy have been verified against plenty of malignancies. However, the oncolytic effects and underlying mechanisms of the recombinant Chinese measles virus vaccine strain Hu191 (rMV-Hu191) against human colorectal cancer (CRC) remain elusive. In this study, the antitumor effects of rMV-Hu191 were evaluated in CRC both in vitro and in vivo. From our data, rMV-Hu191 induced remarkably caspase-dependent apoptosis and complete autophagy in vitro. In mice bearing CRC xenografts, tumor volume was remarkably suppressed and median survival was prolonged significantly with intratumoral treatment of rMV-Hu191. To gain further insight into the relationship of rMV-Hu191-induced apoptosis and autophagy, we utilized Rapa and shATG7 to regulate autophagy. Our data suggested that autophagy was served as a protective role in rMV-Hu191-induced apoptosis in CRC. PI3K/AKT signaling pathway as one of the common upstream pathways of apoptosis and autophagy was activated in CRC after treatment with rMV-Hu191. And inhibition of PI3K/AKT pathway using LY294002 was accompanied by enhanced apoptosis and decreased autophagy which suggested that PI3K/AKT pathway promoted rMV-Hu191-induced autophagy and inhibited rMV-Hu191-induced apoptosis. This is the first study to demonstrate that rMV-Hu191 could be used as a potentially effective therapeutic agent in CRC treatment. As part of the underlying cellular mechanisms, apoptosis and autophagy were involved in the oncolytic effects generated by rMV-Hu191. And the cross-talk between these two processes and the PI3K/AKT signaling pathway was well identified.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Transl Oncol Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Transl Oncol Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos