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A new subspecies of Trypanosoma cyclops found in the Australian terrestrial leech Chtonobdella bilineata.
Ellis, John; Barratt, Joel; Kaufer, Alexa; Pearn, Lauren; Armstrong, Brigette; Johnson, Michael; Park, Yasunori; Downey, Lara; Cao, Maisie; Neill, Levina; Lee, Rogan; Ellis, Bethany; Tyler, Kevin; Lun, Zhao-Rong; Stark, Damien.
Afiliación
  • Ellis J; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Barratt J; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Kaufer A; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Pearn L; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Armstrong B; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Johnson M; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Park Y; School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.
  • Downey L; Department of Microbiology, St Vincent's Hospital, Sydney, Darlinghurst, NSW, Australia.
  • Cao M; Department of Microbiology, St Vincent's Hospital, Sydney, Darlinghurst, NSW, Australia.
  • Neill L; NSW Health Pathology, ICPMR, Westmead Hospital, Westmead, NSW, Australia.
  • Lee R; Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, Westmead Hospital, Westmead, NSW, Australia.
  • Ellis B; Research School of Earth Sciences, Australian National University, Canberra, ACT, Australia.
  • Tyler K; Norwich Medical School, University of East Anglia, Norwich, Norfolk, UK.
  • Lun ZR; Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou510275, China.
  • Stark D; Department of Microbiology, St Vincent's Hospital, Sydney, Darlinghurst, NSW, Australia.
Parasitology ; 148(10): 1125-1136, 2021 09.
Article en En | MEDLINE | ID: mdl-33843511
Previously, it was suggested that haemadipsid leeches represent an important vector of trypanosomes amongst native animals in Australia. Consequently, Chtonobdella bilineata leeches were investigated for the presence of trypanosome species by polymerase chain reaction (PCR), DNA sequencing and in vitro isolation. Phylogenetic analysis ensued to further define the populations present. PCR targeting the 28S rDNA demonstrated that over 95% of C. bilineata contained trypanosomes; diversity profiling by deep amplicon sequencing of 18S rDNA indicated the presence of four different clusters related to the Trypanosoma (Megatrypanum) theileri. Novy­MacNeal­Nicolle slopes with liquid overlay were used to isolate trypanosomes into culture that proved similar in morphology to Trypanosoma cyclops in that they contained a large numbers of acidocalcisomes. Phylogeny of 18S rDNA/GAPDH/ND5 DNA sequences from primary cultures and subclones showed the trypanosomes were monophyletic, with T. cyclops as a sister group. Blood-meal analysis of leeches showed that leeches primarily contained blood from swamp wallaby (Wallabia bicolour), human (Homo sapiens) or horse (Equus sp.). The leech C. bilineata is a host for at least five lineages of Trypanosoma sp. and these are monophyletic with T. cyclops; we propose Trypanosoma cyclops australiensis as a subspecies of T. cyclops based on genetic similarity and biogeography considerations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma / Interacciones Huésped-Parásitos / Sanguijuelas Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: Parasitology Año: 2021 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma / Interacciones Huésped-Parásitos / Sanguijuelas Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: Parasitology Año: 2021 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido