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Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae.
Shin, Minhye; Park, Heeyoung; Kim, Sooah; Oh, Eun Joong; Jeong, Deokyeol; Florencia, Clarissa; Kim, Kyoung Heon; Jin, Yong-Su; Kim, Soo Rin.
Afiliación
  • Shin M; Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Science, Seoul National University, Seoul, South Korea.
  • Park H; School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
  • Kim S; Department of Environment Science and Biotechnology, Jeonju University, Jeonju, South Korea.
  • Oh EJ; Department of Food Science, Purdue University, West Lafayette, IN, United States.
  • Jeong D; School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
  • Florencia C; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
  • Kim KH; Department of Biotechnology, Graduate School, Korea University, Seoul, South Korea.
  • Jin YS; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
  • Kim SR; School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
Front Bioeng Biotechnol ; 9: 654177, 2021.
Article en En | MEDLINE | ID: mdl-33842449
Being a microbial host for lignocellulosic biofuel production, Saccharomyces cerevisiae needs to be engineered to express a heterologous xylose pathway; however, it has been challenging to optimize the engineered strain for efficient and rapid fermentation of xylose. Deletion of PHO13 (Δpho13) has been reported to be a crucial genetic perturbation in improving xylose fermentation. A confirmed mechanism of the Δpho13 effect on xylose fermentation is that the Δpho13 transcriptionally activates the genes in the non-oxidative pentose phosphate pathway (PPP). In the current study, we found a couple of engineered strains, of which phenotypes were not affected by Δpho13 (Δpho13-negative), among many others we examined. Genome resequencing of the Δpho13-negative strains revealed that a loss-of-function mutation in GCR2 was responsible for the phenotype. Gcr2 is a global transcriptional factor involved in glucose metabolism. The results of RNA-seq confirmed that the deletion of GCR2 (Δgcr2) led to the upregulation of PPP genes as well as downregulation of glycolytic genes, and changes were more significant under xylose conditions than those under glucose conditions. Although there was no synergistic effect between Δpho13 and Δgcr2 in improving xylose fermentation, these results suggested that GCR2 is a novel knockout target in improving lignocellulosic ethanol production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Suiza