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Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites.
Chmayssem, Ayman; Verplanck, Nicolas; Tanase, Constantin Edi; Costa, Guillaume; Monsalve-Grijalba, Karen; Amigues, Simon; Alias, Mélanie; Gougis, Maxime; Mourier, Véronique; Vignoud, Séverine; Ghaemmaghami, Amir M; Mailley, Pascal.
Afiliación
  • Chmayssem A; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France. Electronic address: ayman.chmayssem@cea.fr.
  • Verplanck N; Univ. Grenoble Alpes, CEA, LETI, DTBS, LSMB, 38000, Grenoble, France.
  • Tanase CE; Immunology & Immuno-Bioengineering Group, School of Life Sciences, Faculty of Medicine & Health Sciences, University of Nottingham, United Kingdom.
  • Costa G; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Monsalve-Grijalba K; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Amigues S; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Alias M; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Gougis M; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Mourier V; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Vignoud S; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France.
  • Ghaemmaghami AM; Immunology & Immuno-Bioengineering Group, School of Life Sciences, Faculty of Medicine & Health Sciences, University of Nottingham, United Kingdom.
  • Mailley P; Univ. Grenoble Alpes, CEA, LETI, DTBS, L2CB, 38000, Grenoble, France. Electronic address: pascal.mailley@cea.fr.
Talanta ; 229: 122275, 2021 Jul 01.
Article en En | MEDLINE | ID: mdl-33838777
There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Microfluídica Idioma: En Revista: Talanta Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Microfluídica Idioma: En Revista: Talanta Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos