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Continuous anaerobic oxidation of methane: Impact of semi-continuous liquid operation and nitrate load on N2O production and microbial community.
Valenzuela, Edgardo I; Ortiz-Zúñiga, María F; Carrillo-Reyes, Julián; Moreno-Andrade, Iván; Quijano, Guillermo.
Afiliación
  • Valenzuela EI; Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico.
  • Ortiz-Zúñiga MF; Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico.
  • Carrillo-Reyes J; Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico.
  • Moreno-Andrade I; Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico.
  • Quijano G; Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico. Electronic address: GQuijanoG@iingen.unam.mx.
Chemosphere ; 278: 130441, 2021 Sep.
Article en En | MEDLINE | ID: mdl-33838410
This work proves the feasibility of employing regular secondary activated sludge for the enrichment of a microbial community able to perform the anaerobic oxidation of methane coupled to nitrate reduction (N-AOM). After 96 days of activated sludge enrichment, a clear N-AOM activity was observed in the resulting microbial community. The methane removal potential of the enriched N-AOM culture was then studied in a stirred tank reactor (STR) operated in continuous mode for methane supply and semi-continuous mode for the liquid phase. The effect of applying nitrate loads of ∼22, 44, 66, and 88 g NO3- m-3 h-1 on (i) STR methane and nitrate removal performance, (ii) N2O emission, and (iii) microbial composition was investigated. Methane elimination capacities from 21 ± 13.3 to 55 ± 12 g CH4 m-3 h-1 were recorded, coupled to nitrate removal rates ranging from 6 ± 3.2 to 43 ± 14.9 g NO3- m-3 h-1. N2O production was not detected under the three nitrate loading rates applied for the assessment of potential N2O emission in the continuous N-AOM process (i.e. ∼22-66 g NO-3 m-3 h-1). The lack of N2O emissions during the process was attributed to the N2O reducing capacity of the bacterial taxa identified and the rigorous control of dissolved O2 and pH implemented (dissolved O2 values ≤ 0.07 g m-3 and pH of 7.6 ± 0.4). Microbial characterization showed that the N-AOM process was performed in absence of putative N-AOM archaea and bacteria (ANME-2d, M. oxyfera). Instead, microbial activity was driven by methane-oxidizing bacteria and denitrifying bacteria (Bacteroidetes, α-, and γ-proteobacteria).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Metano Idioma: En Revista: Chemosphere Año: 2021 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Metano Idioma: En Revista: Chemosphere Año: 2021 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido