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Correlative Light and Electron Microscopy for Nanoparticle-Cell Interaction and Protein Localization.
Jung, Minkyo; Kim, Tae Keun; Woo, Ha-Na; Mun, Ji Young; Lee, Heuiran; Pack, Chan-Gi.
Afiliación
  • Jung M; Neural Circuits Research Group, Korea Brain Research Institute, Daegu, South Korea.
  • Kim TK; Convergence Medicine Research Center (CREDIT), Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea.
  • Woo HN; Department of Microbiology, University of Ulsan College of Medicine, Seoul, South Korea.
  • Mun JY; Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul, South Korea.
  • Lee H; Neural Circuits Research Group, Korea Brain Research Institute, Daegu, South Korea.
  • Pack CG; Department of Microbiology, University of Ulsan College of Medicine, Seoul, South Korea.
Adv Exp Med Biol ; 1310: 115-132, 2021.
Article en En | MEDLINE | ID: mdl-33834435
Various silica-based fluorescent nanoparticles ((Si-FNP)) with magnetic or metal cores represent a standard class of nanoparticles offering new opportunities for high-resolution cellular imaging and biomedicine applications, such as drug delivery. Their high solubility, homogeneity, biocompatibility, and chemical inertness Si-FNPs make them attractive probes for correlative light and electron microscopy (CLEM) studies, offering novel insights into nanoparticle-cell interactions in detail. In the present chapter, we present a procedure for imaging silica-based fluorescent magnetic core-shell nanoparticles (Si-FMNP) at the single-particle scale in cells. Our method facilitates the acquisition of information on the extracellular and intercellular distribution of nanoparticles and their various interactions with various cellular organelles when cells are cultured and electroporated by NPs. In addition, such information could facilitate the evaluation of the efficacy of nanocarriers designed for drug delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas Idioma: En Revista: Adv Exp Med Biol Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas Idioma: En Revista: Adv Exp Med Biol Año: 2021 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos