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Porcine endometrial 3D co-culture: Morphological changes in 3D endometrium tissues according to hormonal changes.
Kim, Sang-Hwan; Park, Yong-Su; Shin, Da-Hye; Moon, Jeong-Chan; Oh, Min-Gee; Yoon, Jong-Taek.
Afiliación
  • Kim SH; Institute of Genetic Engineering, Hankyong National University, Jungang-ro, Ansung, Gyeonggi-do, Korea.
  • Park YS; Research Center for Endangered Species, National Institute of Ecology, Geumgang-ro, Maseo-myeon, Seocheon-gun, Chungcheongnam-do, Korea.
  • Shin DH; Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong, Gyeonggi-do, Korea.
  • Moon JC; Research Center for Endangered Species, National Institute of Ecology, Geumgang-ro, Maseo-myeon, Seocheon-gun, Chungcheongnam-do, Korea.
  • Oh MG; Department of Animal Life and Environment Science, Hankyong National University, Jungang-ro, Ansung, Gyeonggi-do, Korea.
  • Yoon JT; Department of Animal Life and Environment Science, Hankyong National University, Jungang-ro, Ansung, Gyeonggi-do, Korea.
Histol Histopathol ; 36(8): 833-844, 2021 Aug.
Article en En | MEDLINE | ID: mdl-33829420
Cells cultured as monolayers proliferate well, but do not sustain their differentiation characteristics. Previous studies have investigated the interactions between cells and growth factors or cytokines by establishing either in vivo or in vitro three-dimensional (3D) cultures. Using porcine uterine epithelial cells and endometrial cells, the current study was designed to develop a 3D uterine culture system and investigate the response to hormone treatment. Formation of the 3D uterine model was similar to that of uterus from the group supplemented with calcium and magnesium, and the addition of these ions altered the spectrum of basement membrane degrading enzyme expression and activity. In particular, the epithelial cell junctions in the 3D model most closely resembled those of an actual uterus when the medium was supplemented with calcium and magnesium; the intercellular basement membrane structure was also tall under these conditions. The study confirmed that Casp-3 expression was lowest in the P4 (progesterone) treatment group, and this hormone was the most potent stimulus for formation of the endometrial cell layer. Therefore, the addition of calcium and magnesium plays an important role in the formation of a 3D uterine model, and the addition of P4 hormone mimics uterine thickening by stimulating growth of the epithelial cell layer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progesterona / Células del Estroma / Endometrio / Estradiol Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Histol Histopathol Asunto de la revista: HISTOLOGIA / PATOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: España
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progesterona / Células del Estroma / Endometrio / Estradiol Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Histol Histopathol Asunto de la revista: HISTOLOGIA / PATOLOGIA Año: 2021 Tipo del documento: Article Pais de publicación: España