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Interleukin-6 via Toll-Like Receptor 3 Signaling Attenuates the Expression of Proinflammatory Chemokines in Human Podocytes.
Umetsu, Hidenori; Watanabe, Shojiro; Imaizumi, Tadaatsu; Aizawa, Tomomi; Tsugawa, Koji; Kawaguchi, Shogo; Seya, Kazuhiko; Matsumiya, Tomoh; Tanaka, Hiroshi.
Afiliación
  • Umetsu H; Department of Pediatrics, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Watanabe S; Department of Pediatrics, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Imaizumi T; Department of Vascular Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Aizawa T; Department of Pediatrics, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Tsugawa K; Department of Pediatrics, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Kawaguchi S; Department of Vascular Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Seya K; Department of Vascular Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Matsumiya T; Department of Vascular Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
  • Tanaka H; Department of Pediatrics, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
Kidney Blood Press Res ; 46(2): 207-218, 2021.
Article en En | MEDLINE | ID: mdl-33827102
BACKGROUND: Although toll-like receptor 3 (TLR3) signaling is involved in the development of certain chronic kidney diseases, the specific molecular mechanisms underlying inflammatory reactions via activation of TLR3 signaling in human podocytes remain unclear. Interleukin (IL)-6 is a pleiotropic cytokine associated with innate and adaptive immune responses; however, little is known about the implication of IL-6 via the activation of regional TLR3 signaling in the inflammatory reactions in human podocytes. METHODS: We treated immortalized human podocytes with polyinosinic-polycytidylic acid (poly IC), an authentic viral double-stranded RNA, and assessed the expression of IL-6, monocyte chemoattractant protein-1 (MCP-1), and C-C motif chemokine ligand 5 (CCL5) using quantitative real-time reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. To further elucidate the poly IC-induced signaling pathway, we subjected the cells to RNA interference against IFN-ß and IL-6. RESULTS: We found that the activation of TLR3 induced expression of IL-6, MCP-1, CCL5, and IFN-ß in human podocytes. RNA interference experiments revealed that IFN-ß was involved in the poly IC-induced expression of IL-6, MCP-1, and CCL5. Interestingly, IL-6 knockdown markedly increased the poly IC-induced expression of MCP-1 and CCL5. Further, treatment of cells with IL-6 attenuated the expression of CCL5 and MCP-1 mRNA and proteins. CONCLUSION: IL-6 induced by TLR3 signaling negatively regulates the expression of representative TLR3 signaling-dependent proinflammatory chemokines in human podocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Interleucina-6 / Quimiocinas / Podocitos / Receptor Toll-Like 3 / Inflamación Límite: Humans Idioma: En Revista: Kidney Blood Press Res Asunto de la revista: NEFROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Interleucina-6 / Quimiocinas / Podocitos / Receptor Toll-Like 3 / Inflamación Límite: Humans Idioma: En Revista: Kidney Blood Press Res Asunto de la revista: NEFROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza