Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root.

Zhang, Tian-Qi; Chen, Yu; Liu, Ye; Lin, Wen-Hui; Wang, Jia-Wei

Zhang, Tian-Qi; Chen, Yu; Liu, Ye; Lin, Wen-Hui; Wang, Jia-Wei.

Afiliación

Zhang TQ; National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences (CAS), Shanghai, China. tianqizhang@yeah.net.
Chen Y; National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences (CAS), Shanghai, China.
Liu Y; University of Chinese Academy of Sciences, Shanghai, China.
Lin WH; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Wang JW; Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Joint Center for Single Cell Biology, Shanghai Jiao Tong University, Shanghai, China.

Nat Commun ; 12(1): 2053, 2021 04 06.

Article en En | MEDLINE | ID: mdl-33824350

RESUMEN

Root development relies on the establishment of meristematic tissues that give rise to distinct cell types that differentiate across defined temporal and spatial gradients. Dissection of the developmental trajectories and the transcriptional networks that underlie them could aid understanding of the function of the root apical meristem in both dicots and monocots. Here, we present a single-cell RNA (scRNA) sequencing and chromatin accessibility survey of rice radicles. By temporal profiling of individual root tip cells we reconstruct continuous developmental trajectories of epidermal cells and ground tissues, and elucidate regulatory networks underlying cell fate determination in these cell lineages. We further identify characteristic processes, transcriptome profiles, and marker genes for these cell types and reveal conserved and divergent root developmental pathways between dicots and monocots. Finally, we demonstrate the potential of the platform for functional genetic studies by using spatiotemporal modeling to identify a rice root meristematic mutant from a cell-specific gene cohort.

Asunto(s)

Diferenciación Celular/genética; Cromatina/metabolismo; Oryza/genética; Raíces de Plantas/citología; Análisis de la Célula Individual; Transcriptoma/genética; Arabidopsis/citología; Agregación Celular; Células Epidérmicas/citología; Raíces de Plantas/genética; Raíces de Plantas/crecimiento & desarrollo; Especificidad de la Especie; Transcripción Genética

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Cromatina / Diferenciación Celular / Raíces de Plantas / Análisis de la Célula Individual / Transcriptoma Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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