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Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism.
Nichols, Robert J; LaFrance, Benjamin; Phillips, Naiya R; Radford, Devon R; Oltrogge, Luke M; Valentin-Alvarado, Luis E; Bischoff, Amanda J; Nogales, Eva; Savage, David F.
Afiliación
  • Nichols RJ; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • LaFrance B; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • Phillips NR; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • Radford DR; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Oltrogge LM; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • Valentin-Alvarado LE; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
  • Bischoff AJ; Department of Chemistry, University of California Berkeley, Berkeley, United States.
  • Nogales E; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
  • Savage DF; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States.
Elife ; 102021 04 06.
Article en En | MEDLINE | ID: mdl-33821786
Prokaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle-like compartment in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of encapsulins, bioinformatic evidence suggests that a great diversity of encapsulin nanocompartments remains unexplored. Here, we describe a novel encapsulin in the freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting sequence. Using cryo-electron microscopy, we have determined the structure of the nanocompartment complex to 2.2 Å resolution. Lastly, biochemical characterization of the complex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation. Taken together, our discovery, structural analysis, and enzymatic characterization of this prokaryotic nanocompartment provide a foundation for future studies seeking to understand the physiological role of this encapsulin in various bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azufre / Proteínas Bacterianas / Synechococcus Idioma: En Revista: Elife Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azufre / Proteínas Bacterianas / Synechococcus Idioma: En Revista: Elife Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido