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Increased Gene expression of CCL2/CCR2 axis in bronchial smooth muscles of allergen-challenged mice.
Chiba, Yoshihiko; Okumura, Kaori; Tamaki, Sayuri; Yasuhara, Yurika; Suto, Wataru; Hanazaki, Motohiko; Sakai, Hiroyasu.
Afiliación
  • Chiba Y; Laboratory of Molecular Biology and Physiology, Hoshi University School of Pharmacy, Japan. Electronic address: chiba@hoshi.ac.jp.
  • Okumura K; Laboratory of Molecular Biology and Physiology, Hoshi University School of Pharmacy, Japan.
  • Tamaki S; Laboratory of Molecular Biology and Physiology, Hoshi University School of Pharmacy, Japan.
  • Yasuhara Y; Laboratory of Molecular Biology and Physiology, Hoshi University School of Pharmacy, Japan.
  • Suto W; Laboratory of Molecular Biology and Physiology, Hoshi University School of Pharmacy, Japan.
  • Hanazaki M; Department of Anesthesiology and Intensive Care Medicine, School of Medicine, International University of Health and Welfare, Japan.
  • Sakai H; Laboratory of Biomolecular Pharmacology, Hoshi University School of Pharmacy, Japan.
Respir Physiol Neurobiol ; 289: 103669, 2021 07.
Article en En | MEDLINE | ID: mdl-33813049
PURPOSE: Augmented bronchial smooth muscle (BSM) contraction is a cause of airway hyperresponsiveness (AHR) in asthma. Increasing evidence suggest that C-C motif chemokine 2 (CCL2) modulates smooth muscle contractility by activating its binding partner C-C chemokine receptor type 2 (CCR2). In the present study, changes in the gene expression of CCL2/CCR2 axis were determined in the BSMs of a murine model of allergic asthma. MATERIALS AND METHODS: The ovalbumin (OA)-sensitized mice were repeatedly challenged with aerosolized OA to induce asthmatic reaction. Twenty-four hours after the last antigen challenge, total RNAs of the main BSM tissues and bronchoalveolar lavage fluids (BALFs) were obtained. RESULTS: Our published microarray data (GEO accession No. GSE116504) detected changes in gene expression associated with the chemokine signaling pathway (KEGG Map ID: 04062) in BSMs of mice with AHR induced by antigen exposure. Among them, quantitative RT-PCR analyses showed significant increase in mRNA expression of Ccl2 and Ccr2. Analysis of BALFs also revealed a significant increase in Ccl2 protein in the airways of the diseased animals. CONCLUSION: It is thus possible that, in association with the AHR, the CCL2/CCR2 axis is enhanced in the airways of allergic bronchial asthma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Asma / Factores de Transcripción / Bronquios / Alérgenos / Expresión Génica / Hiperreactividad Bronquial / Proteínas de Unión al ADN / Receptores CCR2 / Complejos de Clasificación Endosomal Requeridos para el Transporte / Músculo Liso Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Respir Physiol Neurobiol Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Asma / Factores de Transcripción / Bronquios / Alérgenos / Expresión Génica / Hiperreactividad Bronquial / Proteínas de Unión al ADN / Receptores CCR2 / Complejos de Clasificación Endosomal Requeridos para el Transporte / Músculo Liso Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Respir Physiol Neurobiol Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos