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CD56, HLA-DR, and CD45 recognize a subtype of childhood AML harboring CBFA2T3-GLIS2 fusion transcript.
Zangrando, Andrea; Cavagnero, Francesca; Scarparo, Pamela; Varotto, Elena; Francescato, Samuela; Tregnago, Claudia; Cuccurullo, Rosanna; Fagioli, Franca; Nigro, Luca Lo; Masetti, Riccardo; Putti, Maria Caterina; Rizzari, Carmelo; Santoro, Nicola; Pession, Andrea; Pigazzi, Martina; Locatelli, Franco; Basso, Giuseppe; Buldini, Barbara.
Afiliación
  • Zangrando A; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Cavagnero F; Pediatric Hemato Oncology, Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy.
  • Scarparo P; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Varotto E; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Francescato S; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Tregnago C; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Cuccurullo R; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Fagioli F; Pediatric Hemato Oncology, Pausillipon Hospital, Naples, Italy.
  • Nigro LL; Pediatric Onco-Hematology, Stem Cell Transplantation and Cellular Therapy Division, Regina Margherita Children's Hospital, Turin, Italy.
  • Masetti R; Center of Pediatric Hemato-Oncology, Azienda Policlinico-OVE, Catania, Italy.
  • Putti MC; Department of Pediatrics, "Lalla Seràgnoli", Hematology-Oncology Unit, Sant'Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
  • Rizzari C; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Santoro N; Pediatric Hematology-Oncology Unit, Department of Pediatrics, University of Milano-Bicocca, MBBM Foundation c/o ASST Monza, Milan, Italy.
  • Pession A; Department of Pediatric Hemato-Oncology, University of Bari, Bari, Italy.
  • Pigazzi M; Department of Pediatrics, "Lalla Seràgnoli", Hematology-Oncology Unit, Sant'Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
  • Locatelli F; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
  • Basso G; Department of Pediatric Hematology and Oncology, Scientific Institute for Research and Healthcare (IRCCS) Childrens' Hospital Bambino Gesù, Sapienza, University of Rome, Rome, Italy.
  • Buldini B; Pediatric Hemato Oncology, Maternal and Child Health Department, University of Padua, Padua, Italy.
Cytometry A ; 99(8): 844-850, 2021 08.
Article en En | MEDLINE | ID: mdl-33811445
The presence of CBFA2T3-GLIS2 fusion gene has been identified in childhood Acute Myeloid Leukemia (AML). In view of the genomic studies indicating a distinct gene expression profile, we evaluated the role of immunophenotyping in characterizing a rare subtype of AML-CBFA2T3-GLIS2 rearranged. Immunophenotypic data were obtained by studying a cohort of 20 pediatric CBFA2T3-GLIS2-AML and 77 AML patients not carrying the fusion transcript. Enrolled cases were included in the Associazione Italiana di Ematologia Oncologia Pediatrica (AIEOP) AML trials and immunophenotypes were compared using different statistical approaches. By multiple computational procedures, we identified two main core antigens responsible for the identification of the CBFA2T3-GLIS2-AML. CD56 showed the highest performance in single marker evaluation (AUC = 0.89) and granted the most accurate prediction when used in combination with HLA-DR (AUC = 0.97) displaying a 93% sensitivity and 99% specificity. We also observed a weak-to-negative CD45 expression, being exceptional in AML. We here provide evidence that the combination of HLA-DR negativity and intense bright CD56 expression detects a rare and aggressive pediatric AML genetic lesion improving the diagnosis performance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Proteínas de Fusión Oncogénica Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Child / Humans Idioma: En Revista: Cytometry A Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Proteínas de Fusión Oncogénica Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Child / Humans Idioma: En Revista: Cytometry A Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos