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Adjusting the Cut-Off and Maximum Pool Size in RT-qPCR Pool Testing for SARS-CoV-2.
Costa, Murilo S; Sato, Hugo I; Rocha, Raissa P; Carvalho, Alex F; Guimarães, Nathalia S; Machado, Elaine L; Alves, Claudia R L; Teixeira, Santuza M R; Takahashi, Ricardo H C; Tupinambás, Unaí; da Fonseca, Flávio G.
Afiliación
  • Costa MS; Graduate Program in Infectology and Tropical Medicine, Medical School, Universidade Federal de Minas Gerais, Belo Horizonte 30130-100, MG, Brazil.
  • Sato HI; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte 31310-260, MG, Brazil.
  • Rocha RP; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte 31310-260, MG, Brazil.
  • Carvalho AF; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte 31310-260, MG, Brazil.
  • Guimarães NS; Graduate Program in Health and Nutrition, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil.
  • Machado EL; Department of Preventive and Social Medicine, Medical School, Universidade Federal de Minas Gerais, Belo Horizonte 30130-100, MG, Brazil.
  • Alves CRL; Department of Pediatrics, Medical School, Universidade Federal de Minas Gerais, Belo Horizonte 30130-100, MG, Brazil.
  • Teixeira SMR; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte 31310-260, MG, Brazil.
  • Takahashi RHC; Department of Mathematics, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.
  • Tupinambás U; Department of Internal Medicine, Medical School, Universidade Federal de Minas Gerais, Belo Horizonte 30130-100, MG, Brazil.
  • da Fonseca FG; Vaccine Technology Center, Universidade Federal de Minas Gerais, Belo Horizonte 31310-260, MG, Brazil.
Viruses ; 13(4)2021 03 26.
Article en En | MEDLINE | ID: mdl-33810324
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to detect SARS-CoV-2 RNA is an essential test to monitor the occurrence of COVID-19. A methodology is proposed for the determination of maximum pool size and adjustments of cut-off values of cycle threshold (Ct in RT-qPCR pool testing, to compensate for the dilution caused by pooling. The trade-off between pool size and test sensitivity is stated explicitly. The procedure was designed to ensure that samples that would be detectable in individual testing remain detectable in pool testing. The proposed relaxation in cut-off is dependent on the pool size, allowing a relatively tight correction to avoid loss of detection of positive samples. The methodology was evaluated in a study of pool testing of adults attending a public emergency care unit, reference for COVID-19 in Belo Horizonte, Brazil, and presenting flu-like symptoms. Even samples on the edge of detectability in individual testing were detected correctly. The proposed procedure enhances the consistency of RT-qPCR pool testing by enforcing that the scales of detectability in pool processing and in individual sample processing are compatible. This may enhance the contribution of pool testing to large-scale testing for COVID-19.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena en Tiempo Real de la Polimerasa / Prueba de Ácido Nucleico para COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena en Tiempo Real de la Polimerasa / Prueba de Ácido Nucleico para COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza