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In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process.
Cunha, Fernando Bento; Pomini, Karina Torres; Plepis, Ana Maria de Guzzi; Martins, Virgínia da Conceição Amaro; Machado, Eduardo Gomes; de Moraes, Renato; Munhoz, Marcelo de Azevedo E Souza; Machado, Michela Vanessa Ribeiro; Duarte, Marco Antonio Hungaro; Alcalde, Murilo Priori; Buchaim, Daniela Vieira; Buchaim, Rogério Leone; Fernandes, Victor Augusto Ramos; Pereira, Eliana de Souza Bastos Mazuqueli; Pelegrine, André Antonio; Cunha, Marcelo Rodrigues da.
Afiliación
  • Cunha FB; Department of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, Brazil.
  • Pomini KT; Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • Plepis AMG; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo (FOB/USP), Bauru 17012-901, SP, Brazil.
  • Martins VDCA; Postgraduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Marília 17525-902, SP, Brazil.
  • Machado EG; Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • de Moraes R; São Carlos Institute of Chemistry, University of São Paulo, USP, São Carlos 13566-590, SP, Brazil.
  • Munhoz MAES; São Carlos Institute of Chemistry, University of São Paulo, USP, São Carlos 13566-590, SP, Brazil.
  • Machado MVR; Department of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, Brazil.
  • Duarte MAH; Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • Alcalde MP; Department of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, Brazil.
  • Buchaim DV; Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • Buchaim RL; Department of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, Brazil.
  • Fernandes VAR; Interunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, Brazil.
  • Pereira ESBM; Department of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, Brazil.
  • Pelegrine AA; Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (FOB/USP), Bauru 17012-901, SP, Brazil.
  • Cunha MRD; Department of Health Science, Unisagrado University Center, Bauru 17011-160, SP, Brazil.
Molecules ; 26(6)2021 Mar 13.
Article en En | MEDLINE | ID: mdl-33805847
Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopolímeros / Regeneración Ósea / Andamios del Tejido Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopolímeros / Regeneración Ósea / Andamios del Tejido Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza