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A multiple ion-uptake phenotyping platform reveals shared mechanisms affecting nutrient uptake by roots.
Griffiths, Marcus; Roy, Sonali; Guo, Haichao; Seethepalli, Anand; Huhman, David; Ge, Yaxin; Sharp, Robert E; Fritschi, Felix B; York, Larry M.
Afiliación
  • Griffiths M; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
  • Roy S; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
  • Guo H; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
  • Seethepalli A; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
  • Huhman D; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
  • Ge Y; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
  • Sharp RE; Division of Plant Sciences and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA.
  • Fritschi FB; Division of Plant Sciences and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA.
  • York LM; Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
Plant Physiol ; 185(3): 781-795, 2021 04 02.
Article en En | MEDLINE | ID: mdl-33793942
Nutrient uptake is critical for crop growth and is determined by root foraging in soil. Growth and branching of roots lead to effective root placement to acquire nutrients, but relatively little is known about absorption of nutrients at the root surface from the soil solution. This knowledge gap could be alleviated by understanding sources of genetic variation for short-term nutrient uptake on a root length basis. A modular platform called RhizoFlux was developed for high-throughput phenotyping of multiple ion-uptake rates in maize (Zea mays L.). Using this system, uptake rates were characterized for the crop macronutrients nitrate, ammonium, potassium, phosphate, and sulfate among the Nested Association Mapping (NAM) population founder lines. The data revealed substantial genetic variation for multiple ion-uptake rates in maize. Interestingly, specific nutrient uptake rates (nutrient uptake rate per length of root) were found to be both heritable and distinct from total uptake and plant size. The specific uptake rates of each nutrient were positively correlated with one another and with specific root respiration (root respiration rate per length of root), indicating that uptake is governed by shared mechanisms. We selected maize lines with high and low specific uptake rates and performed an RNA-seq analysis, which identified key regulatory components involved in nutrient uptake. The high-throughput multiple ion-uptake kinetics pipeline will help further our understanding of nutrient uptake, parameterize holistic plant models, and identify breeding targets for crops with more efficient nutrient acquisition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Transporte Iónico / Raíces de Plantas / Zea mays Idioma: En Revista: Plant Physiol Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Transporte Iónico / Raíces de Plantas / Zea mays Idioma: En Revista: Plant Physiol Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos