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The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis.
Singer, M; Koedooder, R; Bos, M P; Poort, L; Schoenmakers, S; Savelkoul, P H M; Laven, J S E; de Jonge, J D; Morré, S A; Budding, A E.
Afiliación
  • Singer M; Laboratory of Immunogenetics, Department of Medical Microbiology and Infection Control, Amsterdam UMC, location VUmc, Amsterdam, the Netherlands.
  • Koedooder R; Tubascan, Spin-off at the Department of Medical Microbiology and Infection Control, Amsterdam UMC, location VUmc, Amsterdam, the Netherlands.
  • Bos MP; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Erasmus University Medical Center, Wytemaweg 12, Rotterdam, 3015 CN, The Netherlands. r.koedooder@erasmusmc.nl.
  • Poort L; InBiome B.V, Amsterdam, The Netherlands.
  • Schoenmakers S; InBiome B.V, Amsterdam, The Netherlands.
  • Savelkoul PHM; Division Obstetrics, Department of Obstetrics and Gynecology, Erasmus University Medical Centre, Rotterdam, The Netherlands.
  • Laven JSE; Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, the Netherlands.
  • de Jonge JD; Department of Medical Microbiology and Infection Control, Amsterdam UMC, location VUmc, Amsterdam, the Netherlands.
  • Morré SA; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Erasmus University Medical Center, Wytemaweg 12, Rotterdam, 3015 CN, The Netherlands.
  • Budding AE; ARTPred B.V, Seringenstraat 15, 's Hertogenbosch, 5213 GS, The Netherlands.
BMC Microbiol ; 21(1): 100, 2021 03 31.
Article en En | MEDLINE | ID: mdl-33789573
BACKGROUND: 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host. However, compared to other diagnostic techniques, 16S rRNA gene sequencing is fairly costly and labor intensive, leaving room for other techniques to improve on these aspects. RESULTS: The current study aimed to compare the output of 16S rRNA gene sequencing to the output of the quick IS-pro analysis, using vaginal swab samples from 297 women of reproductive age. 16S rRNA gene sequencing and IS-pro analyses yielded very similar vaginal microbiome profiles, with a median Pearson's R2 of 0.97, indicating a high level of similarity between both techniques. CONCLUSIONS: We conclude that the results of 16S rRNA gene sequencing and IS-pro are highly comparable and that both can be used to accurately determine the vaginal microbiota composition, with the IS-pro analysis having the benefit of rapidity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Vagina / Técnicas Bacteriológicas / Microbiota Límite: Adult / Female / Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Vagina / Técnicas Bacteriológicas / Microbiota Límite: Adult / Female / Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido