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The Convoluted Tubules of the Nephron Must Be Considered Elliptical, and Not Circular, in Stereological Studies of the Kidney.
Ortega-Martinez, Marta; Gutierrez-Davila, Vanessa; Gutierrez-Arenas, Esthefania; Niderhauser-Garcia, Alberto; Cerda-Flores, Ricardo M; Jaramillo-Rangel, Gilberto.
Afiliación
  • Ortega-Martinez M; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Mexico.
  • Gutierrez-Davila V; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Mexico.
  • Gutierrez-Arenas E; School of Biology, Autonomous University of Sinaloa, Culiacan, Mexico.
  • Niderhauser-Garcia A; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Mexico.
  • Cerda-Flores RM; School of Nursing, Autonomous University of Nuevo Leon, Monterrey, Mexico.
  • Jaramillo-Rangel G; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Mexico.
Kidney Blood Press Res ; 46(2): 229-235, 2021.
Article en En | MEDLINE | ID: mdl-33789285
INTRODUCTION: The diameter and area of the proximal convoluted tubule (PCT) and the distal convoluted tubule (DCT) are of the main parameters analyzed in stereological studies of the kidney. However, there is no consensus about if the PCT and DCT should be considered circular or elliptical in shape. OBJECTIVE: To analyze if there are significant differences in the diameter and area of the PCT and DCT, depending on whether they are considered circular or elliptical. METHODS: Paraffin-embedded sections of kidneys from CD1 mice were stained with hematoxylin and eosin and examined using a light microscope. Images were captured using a camera linked to image analysis software. A short diameter (d) and a long diameter (D) were measured in both PCT and DCT. A small circular area (SCA), a large circular area (LCA), and an elliptical area (EA) were calculated with mathematical formulas that incorporate d and D values, while a program area (PA) was provided by the software. RESULTS: There was a significant difference between d and D in both PCT (F = 1.354, Sig = 0.000) and DCT (F = 4.989, Sig = 0.000). Also, there were significant differences in the tubular areas in both PCT (F = 34.843, Sig = 0.000) and DCT (F = 22.390, Sig = 0.000); circular areas were different from elliptical areas (SCA and LCA vs. EA and PA). CONCLUSION: The convoluted tubules of the nephron must not be considered circular, but rather elliptical; care should be taken every time the tubules are analyzed in stereological studies of the kidney, especially when evaluating their diameters and areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Túbulos Renales / Nefronas Límite: Animals Idioma: En Revista: Kidney Blood Press Res Asunto de la revista: NEFROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: México Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Túbulos Renales / Nefronas Límite: Animals Idioma: En Revista: Kidney Blood Press Res Asunto de la revista: NEFROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: México Pais de publicación: Suiza