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EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019.
Spano, Liliana Cruz; Guerrieri, Caroline Gastaldi; Volpini, Lays Paula Bondi; Schuenck, Ricardo Pinto; Goulart, Jaqueline Pegoretti; Boina, Elizabeth; Recco, Celia Regina Nascimento; Ribeiro-Rodrigues, Rodrigo; Dos Santos, Luís Fernando; Fumian, Tulio Machado.
Afiliación
  • Spano LC; Department of Pathology, Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil. liliana.spano@ufes.br.
  • Guerrieri CG; Department of Pathology, Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil.
  • Volpini LPB; Department of Pathology, Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil.
  • Schuenck RP; Department of Pathology, Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil.
  • Goulart JP; State Health Secretariat, Central Public Health Laboratory, Vitoria, Espírito Santo, Brazil.
  • Boina E; State Health Secretariat, Central Public Health Laboratory, Vitoria, Espírito Santo, Brazil.
  • Recco CRN; Municipal Health Secretariat, Epidemiology Service, Vila Velha, Espírito Santo, Brazil.
  • Ribeiro-Rodrigues R; Department of Pathology, Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil.
  • Dos Santos LF; State Health Secretariat, Central Public Health Laboratory, Vitoria, Espírito Santo, Brazil.
  • Fumian TM; Adolfo Lutz Institute, Centre of Bacteriology, National Reference Laboratory for Escherichia coli Enteric Infections, São Paulo, Brazil.
BMC Microbiol ; 21(1): 95, 2021 03 29.
Article en En | MEDLINE | ID: mdl-33781202
BACKGROUND: This study describes the investigation of an outbreak of diarrhea, hemorrhagic colitis (HC), and hemolytic uremic syndrome (HUS) at a daycare center in southeastern Brazil, involving fourteen children, six staff members, six family members, and one nurse. All bacterial and viral pathogens detected were genetically characterized. RESULTS: Two isolates of a strain of enterohemorrhagic Escherichia coli (EHEC) serotype O111:H8 were recovered, one implicated in a case of HUS and the other in a case of uncomplicated diarrhea. These isolates had a clonal relationship of 94% and carried the stx2a and eae virulence genes and the OI-122 pathogenicity island. The EHEC strain was determined to be a single-locus variant of sequence type (ST) 327. EHEC isolates were resistant to ofloxacin, doxycycline, tetracycline, ampicillin, and trimethoprim-sulfamethoxazole and intermediately resistant to levofloxacin and ciprofloxacin. Rotavirus was not detected in any samples, and norovirus was detected in 46.7% (14/30) of the stool samples, three of which were from asymptomatic staff members. The noroviruses were classified as the recombinant GII.4 Sydney [P16] by gene sequencing. CONCLUSION: In this outbreak, it was possible to identify an uncommon stx2a + EHEC O111:H8 strain, and the most recent pandemic norovirus strain GII.4 Sydney [P16]. Our findings reinforce the need for surveillance and diagnosis of multiple enteric pathogens by public health authorities, especially during outbreaks.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brotes de Enfermedades / Infecciones por Caliciviridae / Norovirus / Infecciones por Escherichia coli / Escherichia coli Enterohemorrágica Tipo de estudio: Prognostic_studies Límite: Child, preschool / Female / Humans / Infant / Male País/Región como asunto: America do sul / Brasil Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brotes de Enfermedades / Infecciones por Caliciviridae / Norovirus / Infecciones por Escherichia coli / Escherichia coli Enterohemorrágica Tipo de estudio: Prognostic_studies Límite: Child, preschool / Female / Humans / Infant / Male País/Región como asunto: America do sul / Brasil Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido