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Properties of multivesicular release from mouse rod photoreceptors support transmission of single-photon responses.
Hays, Cassandra L; Sladek, Asia L; Field, Greg D; Thoreson, Wallace B.
Afiliación
  • Hays CL; Truhlsen Eye Institute and Department of Ophthalmology and Visual Sciences, College of Medicine, University of Nebraska Medical Center, Omaha, United States.
  • Sladek AL; Cellular and Integrative Physiology, Omaha, United States.
  • Field GD; Pharmacology and Experimental Neuroscience, Omaha, United States.
  • Thoreson WB; Department of Neurobiology, Duke University School of Medicine, Durham, United States.
Elife ; 102021 03 26.
Article en En | MEDLINE | ID: mdl-33769285
Vision under starlight requires rod photoreceptors to transduce and transmit single-photon responses to the visual system. Small single-photon voltage changes must therefore cause detectable reductions in glutamate release. We found that rods achieve this by employing mechanisms that enhance release regularity and its sensitivity to small voltage changes. At the resting membrane potential in darkness, mouse rods exhibit coordinated and regularly timed multivesicular release events, each consisting of ~17 vesicles and occurring two to three times more regularly than predicted by Poisson statistics. Hyperpolarizing rods to mimic the voltage change produced by a single photon abruptly reduced the probability of multivesicular release nearly to zero with a rebound increase at stimulus offset. Simulations of these release dynamics indicate that this regularly timed, multivesicular release promotes transmission of single-photon responses to post-synaptic rod-bipolar cells. Furthermore, the mechanism is efficient, requiring lower overall release rates than uniquantal release governed by Poisson statistics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Células Fotorreceptoras Retinianas Bastones / Fotones Límite: Animals Idioma: En Revista: Elife Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Células Fotorreceptoras Retinianas Bastones / Fotones Límite: Animals Idioma: En Revista: Elife Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido