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Altered inflammasome activation in neonatal encephalopathy persists in childhood.
Kelly, L A; O'Dea, M I; Zareen, Z; Melo, A M; McKenna, E; Strickland, T; McEneaney, V; Donoghue, V; Boylan, G; Sweetman, D; Butler, J; Vavasseur, C; Miletin, J; El-Khuffash, A F; O'Neill, L A J; O'Leary, J J; Molloy, E J.
Afiliación
  • Kelly LA; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • O'Dea MI; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • Zareen Z; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • Melo AM; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • McKenna E; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • Strickland T; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • McEneaney V; Children's Hospital Ireland (CHI) at Tallaght, Dublin, Ireland.
  • Donoghue V; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • Boylan G; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • Sweetman D; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • Butler J; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • Vavasseur C; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • Miletin J; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • El-Khuffash AF; Discipline of Paediatrics, Trinity College, University of Dublin, Dublin, Ireland.
  • O'Neill LAJ; Trinity Translational Medicine Institute (TTMI), Trinity College Dublin and Trinity Research in Childhood Centre (TRiCC), Dublin, Ireland.
  • O'Leary JJ; Radiology, National Maternity Hospital, Dublin, Ireland.
  • Molloy EJ; Department of Pediatrics and Child Health, University College Cork, Cork, Ireland.
Clin Exp Immunol ; 205(1): 89-97, 2021 07.
Article en En | MEDLINE | ID: mdl-33768526
Neonatal encephalopathy (NE) is characterized by altered neurological function in term infants and inflammation plays an important pathophysiological role. Inflammatory cytokines interleukin (IL)-1ß, IL-1ra and IL-18 are activated by the nucleotide-binding and oligomerization domain (NOD)-, leucine-rich repeat domain (LRR)- and NOD-like receptor protein 3 (NLRP3) inflammasome; furthermore, we aimed to examine the role of the inflammasome multiprotein complex involved in proinflammatory responses from the newborn period to childhood in NE. Cytokine concentrations were measured by multiplex enzyme-linked immunosorbent assay (ELISA) in neonates and children with NE in the absence or presence of lipopolysaccharide (LPS) endotoxin. We then investigated expression of the NLRP3 inflammasome genes, NLRP3, IL-1ß and ASC by polymerase chain reaction (PCR). Serum samples from 40 NE patients at days 1 and 3 of the first week of life and in 37 patients at age 4-7 years were analysed. An increase in serum IL-1ra and IL-18 in neonates with NE on days 1 and 3 was observed compared to neonatal controls. IL-1ra in NE was decreased to normal levels at school age, whereas serum IL-18 in NE was even higher at school age compared to school age controls and NE in the first week of life. Percentage of LPS response was higher in newborns compared to school-age NE. NLRP3 and IL-1ß gene expression were up-regulated in the presence of LPS in NE neonates and NLRP3 gene expression remained up-regulated at school age in NE patients compared to controls. Increased inflammasome activation in the first day of life in NE persists in childhood, and may increase the window for therapeutic intervention.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encefalopatías / Inflamasomas / Inflamación Tipo de estudio: Clinical_trials Límite: Child / Child, preschool / Female / Humans / Male / Newborn Idioma: En Revista: Clin Exp Immunol Año: 2021 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encefalopatías / Inflamasomas / Inflamación Tipo de estudio: Clinical_trials Límite: Child / Child, preschool / Female / Humans / Male / Newborn Idioma: En Revista: Clin Exp Immunol Año: 2021 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Reino Unido