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High-efficiency prime editing with optimized, paired pegRNAs in plants.
Lin, Qiupeng; Jin, Shuai; Zong, Yuan; Yu, Hong; Zhu, Zixu; Liu, Guanwen; Kou, Liquan; Wang, Yanpeng; Qiu, Jin-Long; Li, Jiayang; Gao, Caixia.
Afiliación
  • Lin Q; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Jin S; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Zong Y; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Yu H; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Zhu Z; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Liu G; State Key Laboratory of Plant Genomics, and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Kou L; State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Wang Y; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Qiu JL; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
  • Li J; State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • Gao C; State Key Laboratory of Plant Genomics, and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
Nat Biotechnol ; 39(8): 923-927, 2021 08.
Article en En | MEDLINE | ID: mdl-33767395
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / ARN Guía de Kinetoplastida / ARN de Planta / Edición Génica Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / ARN Guía de Kinetoplastida / ARN de Planta / Edición Génica Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos